Are you interested in doing your master thesis at our institute?


We can offer you the following topics and happily receive your request:

 

 

Pharmacological evaluation of dual inhibitors inspired by vitamin E derivatives

Language: English, German
Discovery of novel vitamin E derivatives that dually inhibit 5-LOX and mPGES-1 as potential drug candidates that combine potent anti-inflammatory activity with low side effects. To obtain insights into the pharmacological profile, our in-house compound library is characterized using enzyme activity assays and cell-based studies on human primary immune cells.
Methods:
Human primary immune cell isolation, macrophage polarization, cell cultivation, isolated enzyme assay, solid and liquid phase extraction, LC-MS/MS data analysis.
Supervisor: Permann Stephan




Potential of ω3 fatty acid-containing phosphatidylcholines in the supportive therapy of leukemia and lymphoma

Language: English, German
Polyunsaturated fatty acid-containing phosphatidylcholines (PUFA-PCs) seem to reduce the viability of leukemia and lymphoma cells and may support the traditional therapy of cytoskeleton targeting agents. Further investigation is ongoing whether the PUFA-PCs show the ability to inhibit tumor growth in vivo and support is needed to clarify the influence on different processes in the organism.
Methods:
protein expression (Western Blot), lipid profile of phospholipids, oxidized phospholipids and free fatty acid (UPLC-MS/MS analysis; extraction and analysis of different tissues), determination of reactive oxygen species in tumor tissue, location determination of cytoskeleton relevant compartments (immunofluorescence staining).
Supervisor: Witt Finja




Biological evaluation of potential anti-inflammatory/proresolving agents

Language: English, German
This project aims to discover novel compounds that beneficially effect lipid mediator profiles in vitro and to gain insights into their mode of action.
Methods:
isolation of human primary immune cells, stimulation of immune cells, sample preparation via solid-phase-extraction, analysis of UPLC-ESI-MS/MS data as well as studies with recombinant enzymes.
Supervisor: Waltl Lorenz




Project 1:
Unraveling the molecular mechanism of natural products and synthetic compounds targeting inflammation and ferroptosis
Project 2:
Impact of the immune microenvironment on ferroptotic cancer cell death

Language: English

Project 1: This project aims to characterize novel natural products or synthetic compounds that: 1) limit inflammation in various cell-free or cellular systems and in vivo inflammatory models; 2) target ferroptosis in cancer cells of different origin.
Project 2:This project aims to investigate the communication between the immune microenvironment and cancer cells, with focus on immune cell function and the regulation on ferroptotic cancer cell death.
Methods:
Cell culture of tumor cell lines and primary immune cells; protein expression by Western blot; lipid extraction by solid phase or liquid-liquid extraction; exploration of ferroptosis-regulating pathways (i.e, Fenton reaction activity, labile iron level, GSH/GSSG concentration, GPX activity); analysis of lipid mediators, phospholipids and oxidized phospholipids by UPLC-ESI-MS/MS.
Supervisor: Rao Zhigang




Silymarin-Mediated Inhibition of Acquired Chemotherapy-Resistance in Osteosarcoma

Language: English
An aggressive malignant neoplasm Osteosarcoma (OS) develops from transformed cells of mesenchymal origin illustrating osteoblastic differentiation and mainly arise in the long bones of the limbs. Although substantial achievements owing to chemotherapeutic drugs development have been obtained in treating various cancers including OS, resistance to these drugs followed by metastatic spread are the most important mechanisms responsible for the failure of current therapeutic programs. Preventive/therapeutic strategies overcoming resistance induction are therefore on high demand for different type of cancers including OS. Herbal metabolites such as flavonoids, reveal promising cytoprotective effects, thus providing health benefits. Based on previous data, we aim at specifically targeting chemotherapy-dependent resistance mechanisms using silymarin (standardized extract of Silybum marianum (L.) Gaertn) or its active components.
Methods:
1- Cell culture (drug-resistance establishment, inhibitor treatments, etc.), 2- MTT assay, 3- Proliferation assay, 4- Crystal violet staining, 5- Annexin V/PI staining, 6- Western blotting.
Supervisor: Bonyadirad Ehsan




Ferroptosis-modulating natural products

Language: English, German
Ferroptosis-inhibiting compounds protect non-malignant cells from pathological cell death during (neuro)degenerative diseases and organ injuries while ferroptosis-inducing compounds are cytotoxic and induce cancer cell death. Identification of potential novel lead structures either for the treatment of neurodegenerative diseases and organ injuries (anti-ferroptotic compounds) or cancer (pro-ferroptotic compounds).
Methods:
During the master thesis, selected natural products will be investigated for their ferroptosis-modulating properties. Most promising candidates will be chosen for further investigations on the molecular mode of action (e.g., GPX4 activity, NRF2 activation, labile iron levels, GSH/GSSG level).
Supervisor: Koeberle Solveigh




If interested please contact Michael-Popp-Forschungsinstitut@uibk.ac.at

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