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Lab for Bioanalytic & Intermediary Metabolism

Biological systems are not only very complex, but also highly dynamic. The dynamic interactions between the metabolome and the proteome are of particular importance for the formation of the phenotype of biological systems. The exact chemical composition of a protein species defines its function, and virtually all biological processes are regulated by posttranslational modifications (PTMs) such as phosphorylation and acetylation.

Our research interests lie in the development of novel hyphenated mass spectrometric techniques for combinatorial time-resolved analysis of the proteo-metabolome and posttranslational modifications (Proteo-Metabo-Flux), functional proteo-metabolomics and fluxomics, spatial OMICs, as well as single-cell proteo-metabolomics to decipher molecular mechanisms at the interface of intermediary metabolism and modified protein species in context of systems biology, epigenetics and tuberous sclerosis complex (TSC) disease.

Research Interest

• Proteo-Metabo-Flux: Investigation of acetylation and methylation dynamics by combined metabolic and chemical labeling (CoMetChem)
• Functional Proteo-Metabolomics and Fluxomics: Development of hyphenated LC-MS techniques and bioinformatic approaches to study the regulatory interplay between cellular intermediary metabolism and modified protein species in metabolic and epigenetic regulation in the context of tuberous sclerosis complex (TSC) disease
• Single-Cell Proteo-Metabolomics
• Top-Down-Proteomics
• Spatial Multi-OMICs

Key technologies 

• Ultrahigh resolution mass spectrometry
• Ion mobility mass spectrometry
• Multi-dimensional chromatography
• Simultaneous Proteo-Metabolomics
• Bioinformatics, computational mass spectrometry
• Laser ablation-based sample preparation

Motivated Bachelor and Master students interested in proteomics, metabolomics and analytical biochemistry are invited to apply for internships or thesis projects. Apply at marcel.kwiatkowski@uibk.ac.at.

Recent Grant Support

• Austrian Centre of Industrial Biotechnology (2024 - 2025)
• Tyrolean Science Fund (2021 – 2023)
• University of Innsbruck-Nachwuchsförderung (2020 - 2022)
• Noordelijke Cara Stichting, Groningen, The Netherlands (2018 - 2019)
• ERS/EU RESPIRE3 Marie Skłodowska-Curie Postdoctoral research fellowship, European Union Horizon 2020 No. 713406 (2017 – 2019)
• FFMTHH, Hamburg, Germany (2015 – 2018)
• FFM, University Medical Center-Eppendorf Hamburg, Germany (2016)