Course Listing and Outline

LECTURE COURSE NUMBER INSTRUCTOR
Biochemistry I 712001 Stefan
Biochemistry II 712002 Stefan
Biochemical Methods 712005 HartlSchneider
Biochemistry and Molecular Biology for Pharmacists 740550 Hartl
Advanced Biochemistry I 712003 Hartl
Advanced Biochemistry II 712004 Hartl
Eucaryotic Gene Regulation 712901 Hartl
Recombinant Protein Technology 712902 Hartl
Protein-Protein and Protein-DNA Interaction 712903 Hartl
Regulation of Cell Proliferation and Effects of Drugs 712904 Hartl
Biochemical Seminar 712905, 712906 HartlSchneider, Stefan, Bister 


LAB COURSE NUMBER INSTRUCTOR
Biochemical Methods
712006 HartlSchneiderStefan
Laboratory Course in Basic Biochemistry 712007 HartlStefan
Laboratory Course in Biochemistry and Molecular Biology for Pharmacists 740502, 740551 HartlSchneiderStefan
Laboratory Course in Advanced Biochemistry 712012, 712013 HartlSchneiderStefan
Specialized Laboratory Course in Biochemistry 712014, 712015 HartlSchneiderStefan

 

TEACHING SERVICES FOR THE FACULTY OF BIOLOGY NUMBER INSTRUCTOR
Biochemistry for Biologists 712050 Schneider
Laboratory Course in Basic Biochemistry 712008, 712009 Schneider
Biochemistry for Prospective Teachers 719610 Schneider

 

Biochemistry I

A Chemical Components of the Cell

1. Subject and Unifying Principles of Biochemistry
2. Water, Acids and Bases
3. Amino Acids, Peptides, and Proteins
4. Structure and Function of Proteins
5. Structure and Function of Enzymes
6. Vitamines and Coenzymes
7. Lipids
8. Carbohydrates
9. Nucleic Acids

B Metabolism

10. Principal Metabolic Strategies
11. Glycolysis
12. Citric Acid Cycle
13. Oxidative Phosphorylation
14. Pentose Phosphate Pathway
15. Gluconeogenesis
16. Glycogen Metabolism
17. Fatty Acid Metabolism
18. Amino Acid Degradation

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Biochemistry II

B Metabolism (continued)

19. Photosynthesis
20. Biosynthesis of Complex Lipids and Steroids
21. Biosynthesis of Amino Acids
22. Biosynthesis and Degradation of Heme
23. Biosynthesis and Degradation of Nucleotides
24. Coordination of Metabolism

C Biochemistry and Molecular Genetics

25. Structure and Replication of DNA
26. Transcription and RNA Processing
27. Protein Synthesis
28. Regulation of Gene Expression in Prokaryotes
29. Eukaryotic Chromosomes and Gene Expression
30. Gene Technology

D Biochemical Basis of Complex Biological Processes

31. Molecular Immunology
32. Muscle Contraction and Motility
33. Hormone Function
34. Signal Transduction
35. Membrane Transport

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Biochemical Methods

1. Preparation and Electrophoresis of Nucleic Acids
2. Hybridization and Detection of Nucleic Acids
3. Gene Libraries and Genome Analysis
4. Monitoring of Differential Gene Activity
5. Analysis of Promoters and of Chromatin
6. DNA Sequencing and Bioinformatic Sequence Analysis
7. Preparation and Analytics of Recombinant Proteins
8. Mass Spectrometry of Proteins
9. Spectroscopical Methods
10. X-ray Structure Analysis of DNA and Proteins
11. Nuclear Magnetic Resonnance Spectroscopy of Proteins
12. Electron Microscopic Investigations
13. Analysis of Protein-Nucleic Acid Interactions
14. Analysis of Protein-Protein Interactions
15. Immunological Techniques for Protein Detection
16. Analysis of Cellular Proteins
17. Cell Biological Methods
18. Exploring Mechanisms of Signal Transduction
19. Gene Modification and Reverse Genetics
20. Specific Gene Inactivation

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Biochemistry and Molecular Biology for Pharmacists

A) Molecular Design of Life

1. Biochemistry: an Overview
2. Protein Structure and Function
3. Protein and Proteome Analysis
4. Nucleic Acids and Genetic Flow
5. Evolution and Bioinformatics
6. Enzymes and Inhibitors
7. Vitamins and Coenzymes
8. Carbohydrates and Glycosylation
9. Lipids and Cell Membranes
10. Metabolism: Concepts and Design

B) Generation and Storage of Energy

11. Glycolysis and Gluconeogenesis
12. Citric acid Cycle and Respiration
13. Photosynthesis
14. Pentose Phosphate Pathway
15. Glycogen Metabolism
16. Fatty acid Metabolism
17. Amino Acid Metabolism
18. Nucleotide Metabolism
19. Synthesis of Complex Lipids
20. Synthesis of Steroids

C) Molecular Biology and Complex Systems

21. DNA Replication, Recombination, Repair
22. RNA Synthesis and Processing
23. Protein Biosynthesis
24. Gene Expression Control
25. Gene and Genome Analysis
26. Signal Transduction
27. Sensory Systems
28. Immune System
29. Molecular Motors
30. Drug Development

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Advanced Biochemistry I

An Advanced Treatment of:

1. Amino Acids
2. Peptide Bond
3. Protein Conformation
4. Protein Analysis
5. Allosteric Proteins
6. Mechanisms of Enzymatic Catalysis
7. Protein Sequence Motifs
8. Protein Folding
9. Protein Degradation
10. Protein Function

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Advanced Biochemistry II

An Advanced Treatment of:

1. Amino Acid Metabolism
2. Regulation of Cholesterol Metabolism
3. Steroid Hormones / Isoprenoid Compounds
4. Structure and Chemical Attributes of DNA
5. Gene-Protein Relation
6. Protein Targeting
7. Mechanisms of Gene Regulation
8. Signal Transduction

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Eucaryotic Gene Regulation

An Advanced Treatment of:

1. Nucleic Acid Hybridization
2. Gene Libraries and Gene Mapping
3. Differential Gene Expression
4. Promoter Analysis
5. Chromatin Analysis
6. Bioinformatic Sequence Analysis

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Recombinant Protein Technology

An Advanced Treatment of:

1. Recombinant Proteins and Synthetic Peptides
2. Molecular Separation Techniques
3. Mass Spectrometry of Proteins
4. Spectroscopic Methods
5. X-ray Analysis of DNA and Proteins
6. Nuclear Magnetic Resonance (NMR) Spectroscopy of Proteins 

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Protein-Protein and Protein-DNA Interaction

An Advanced Treatment of:

1. Preparation of Nucleic Acids
2. Radioactive Labeling of DNA
3. Analysis of Protein-Protein Interactions
4. Analysis of Protein-DNA Interactions
5. Protein Analysis using Immunological Techniques
6. Electron Microscopy

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Regulation of Cell Proliferation and Effects of Drugs

An Advanced Treatment of:

1. Gene Modifications
2. Targeted Gene Silencing
3. Cell Biological Methods
4. Cell Systems
5. Molecular Mechanisms of Cell Proliferation
6. Drug Development

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Biochemical Seminar

Presentation and discussion of current research results and biochemical topics.

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Laboratory Course in Basic Biochemistry 

Experiment Topic Methodology
1 Preparation of high molecular weight DNA Isolation of eukaryotic genomic DNA, photometric analysis of nucleic acids
2 Molecular cloning and sequence analysis of DNA Isolation of plasmid DNA, restriction enzyme digestion of DNA and agarose gel electrophoresis, cycle sequencing of DNA
3 Synthesis and modification of DNA Polymerase chain reaction (PCR), nucleic acid hybridization, radioactive end-labeling of DNA
4 DNA-protein interaction Electrophoretic mobility shift assay (EMSA), native polyacrylamide gel electrophoresis, phosphor imaging
5 Protein-protein interaction Computerized protein sequence analysis, sequence alignments, protein fragment complementation assay, bioluminescence readout
6 RNA preparation Isolation of total RNA from eukaryotic cells, preparative ultracentrifugation
7 Differential gene expression analysis Reverse transcription of mRNA, PCR amplification of cDNA, agarose gel electrophoresis, real-time PCR
8 Protein expression and purification Recombinant protein expression, affinity chromatography, fast protein liquid chromatography (FPLC)
9 Protein analysis SDS polyacrylamide gel electrophoresis, protein staining, molecular weight determination
10 Protein identification Immunoblotting of proteins, detection by reporter enzymes coupled to antibodies


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Laboratory Course in Biochemistry and Molecular Biology for Pharmacists 

Experiment Topic Methodology
1 Preparation of high molecular weight DNA Isolation of eukaryotic genomic DNA, photometric analysis of nucleic acids
2 Molecular cloning and sequence analysis of DNA Isolation of plasmid DNA, restriction enzyme digestion of DNA and agarose gel electrophoresis, cycle sequencing of DNA
3 Differential gene expression analysis Reverse transcription of mRNA, PCR amplification of cDNA, agarose gel electrophoresis, real-time PCR
4 Protein expression and purification Recombinant protein expression, affinity chromatography, fast protein liquid chromatography (FPLC)
5 Protein analysis SDS polyacrylamide gel electrophoresis, protein staining, molecular weight determination


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Laboratory Course in Advanced Biochemistry

Students taking this course will participate in current research projects and will receive hands-on training in modern techniques in the fields of biochemistry, molecular biology, and gene technology. Students taking the lab course have also to enroll in the parallel introductory lecture course (712.015). Both courses are offered year-round and registration is possible any time. Prospective participants must have completed the lecture and laboratory courses in basic biochemistry.

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Specialized Laboratory Course in Biochemistry

Participation in challenging research projects at an advanced stage.

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