Phenotypical assessment of Plasmodiophora brassicae infection of different Arabidopsis arenosa genotypes: detection of similarities and differences among the parasite-host interaction

 

Student/in: Sabine Oberhofer, BSc
Termin: 25.07.2023, 10:00 Uhr
Ort: HS F, Technik

1. Prüfer/in: Univ.-Prof. Mag. Dr. Sigrid Neuhauser
2. Prüfer/in: Nadine Präg, MSc, PhD
Vorsitzende/r: Priv.-Doz. Mag. Dr. Andreas Wagner

 

Interessierte Kolleginnen und Kollegen sind herzlich willkommen!

 

Abstract

Plasmodiophora brassicae is a soil-borne obligate parasite (Kageyama and Asano, 2009), which causes clubroot disease in brassicas (Faggian and Strelkov, 2009). Infections with P. brassicae can lead to gall formation in the roots and hypocotyl, causing reduced growth of the host plant (Faggian and Strelkov, 2009) and annual crop losses of 10 % worldwide (Dixon, 2006). It is assumed that all members of the Brassicaceae family can be potential hosts for P. brassicae (Dixon, 2009). Arabidopsis arenosa (Brassicaceae) is a representative of the model genus Arabidopsis with diploid and autotetraploid populations, living in alpine and lowland areas and showing pronounced phenotypical differences among them (Monnahan et al., 2019). The aim of this master thesis is the phenotypical characterization of the parasite-host interactions of P. brassicae with different ecotypes of A. arenosa. Phenotypical data were generated by infecting different A. arenosa ecotypes with P. brassicae. Additionally, molecular and physiological methods, such us PCR and qPCR, and hormone measurements via GC-MS; were performed to quantify and investigate the infection process. This presentation gives first insights on the phenotypical characterisation of P. brassicae infection on wild populations of A. arenosa.