Anti-fungale Photoaktivitätstests gegen Pflanzenpathogene von Vitis vinifera

 

Student/in: Christina Schweiger, BSc
Termin: 05.09.2024, 09.30 Uhr
Ort: Seminarraum Biologie (Foyer)

1. Prüfer/in: Ao. Univ.-Prof. Mag. Dr. Ursula Peintner
2. Prüfer/in: Mag. Dr. Hermann Strasser​​​​​​​
Vorsitzende/r: Univ.-Prof. Mag. Dr. Rosa Margesin

 

Interessierte Kolleginnen und Kollegen sind herzlich willkommen!

 

Abstract

The high use of conventional antimicrobial substances against fungal plant pathogens in agriculture has contributed significantly to the emergence of resistant plant pathogens, which makes the search for new antimicrobial substances all the more important (Braga et al. 2022; Sundin and Wang 2018). Grapevine (Vitis vinifera) is economically one of the world's most important fruit and is also not spared from the development of resistant plant pathogens (Torregrosa et al. 2015; Kunova et al. 2021; Ambrosini et al. 2020a). This makes it an interesting model organism for new therapeutic methods, such as antimicrobial photodynamic treatment (APDT) (Ambrosini et al. 2020b). Antimicrobial photodynamic treatment (APDT) is a method for treating local infections caused by pathogenic microorganisms. It is based on three basic components: a photosensitizer (PS), light and molecular oxygen (Braga et al. 2022; do Prado-Silva et al. 2022). In the presence of molecular oxygen, the PS when irradiated with light, of the appropriate wavelength, produces reactive oxygen species (ROS). ROS cause damage to the target cells, which in turn can lead to cell death (do Prado-Silva et al. 2022; Ambrosini et al. 2020a). Photosensitizers can be of various natural origins, such as from plants; microorganisms and also fungi (Fiala et al. 2021; Braga et al. 2022). The antifungal photoactivity tests based on the APDT are performed on three different fungal pathogens of Vitis vinifera: Penicillium expansum, Botrytis cinerea and Phomopsis viticola. In the assay curcumin, emodin, aloe-emodin and chrysophanol are used as photosensitzers.