Projekte

Optimization of a genetic tool box for Trichoderma

 

PI: Clara Baldin

 

The overuse of pesticides and antibiotics has contributed to the rise of antimicrobial resistance in various organisms. Beyond the implications for medically relevant pathogens, Trichoderma fungi highlight another aspect of this problem. Despite their importance in sustainable agriculture, Trichoderma species are challenging to genetically manipulate and characterize as, being in several cases isolates from heavily treated fields, they exhibit resistance to several antifungals commonly used in laboratories.

The aim of this project is to develop new genetic tools to facilitate the manipulation and characterization of Trichoderma species, by identifying and validating negative selectable markers and optimizing other molecular strategies. Currently, the following line of research are under investigation:

• Identifying and testing of constitutive and inducible promoters
• Identifying and testing of different fluorescent proteins
• Identification of new genetic markers