Margit Christine Egg

Molecular Lab Manager, head of the molecular core facilities

Margit Christine Egg


Address:
Institute of Zoology
University of Innsbruck

Technikerstr. 25
6020 Innsbruck
Austria

Room: 6/20
Phone: +43 512 507-51865
e-mail: margit.egg@uibk.ac.at

Current Research

Our research focusses on the bi-directional interaction between the circadian clock and the hypoxic pathway of zebrafish larvae and cells. Low oxygen availability thus decreases the oscillation amplitude of the circadian clock on the physiological as well as on the molecular level. In turn, the hypoxic response of the animals depends on day time and is therefore clearly regulated by the circadian clock. The molecular basis behind the circadian regulation of the hypoxic response in zebrafish and the effect of a corrupted clock (as it is the case during jet lag or shiftwork) on the capacity to cope with low oxygen tensions are the questions currently under investigation. Beside hypoxia other timing cues such as light, physical activity or magnetism are tested for their impact on the circadian clock and the hypoxic pathway. 

Margit Egg Collage
©BfÖ 2013 / Anil Ramalingam

For more details see:

Curriculum vitae

1999 - 2002 Scientific assistant at the University hospital for Internal Medicine, Department of Hepatology and Gastroenterology
2002 - 2006 Post Doc at the Institute of Zoology and Limnology, University of Innsbruck
March 2006 Research assistant at the Institute of Zoology and Limnology

 

Projects

  • Auswirkung der Kernspinresonanz Therapie NMR auf die zirkadiane Uhr und den Hypoxie – Signalweg; Funding: MEDTec GmbH, Germany; Duration: 15.05.2015 until 14.05.2018
  • Metallothioneins and their genes in Helix pomatia. Reinhard Dallinger & Margit Egg, FWF, Duration: 2007-2010


Publications

Contributions to Books / Journals

Journal Article (Original Paper)
  • Tischler, A.; Egg, M.; Füreder, L. (2016): Ethanol: A simple and effective RNA-preservation for freshwater insects living in remote habitats.
    In: LIMNOLOGY AND OCEANOGRAPHY - METHODS 14/3, pp. 186 - 195. (DOI)

  • Pelster, B.; Egg, M. (2015): Multiplicity of Hypoxia-Inducible Transcription Factors and Their Connection to the Circadian Clock in the Zebrafish.
    In: PHYSIOLOGICAL AND BIOCHEMICAL ZOOLOGY 88/2, pp. 146 - 157. (DOI)

  • Egg, M.; Paulitsch, M.; Ennemoser, Y.; Wüstenhagen, A.; Schwerte, T.; Sandbichler, A. M.; Fiechtner, B.; Köblitz, L.; Prem, C.; Pelster, B. (2014): Chronodisruption increases cardiovascular risk in zebrafish via reduced clearance of senescent erythrocytes.
    In: CHRONOBIOLOGY INTERNATIONAL 31/5, pp. 680 - 689. (DOI)

  • Kopp, R.; Bauer, I.; Ramalingam, A.; Egg, M.; Schwerte, T. (2014): Prolonged Hypoxia Increases Survival Even in Zebrafish (Danio rerio) Showing Cardiac Arrhythmia.
    In: PLoS One 9/2, p. e89099. (DOI)

  • Egg, M.; Köblitz, L.; Hirayama, J.; Schwerte, T.; Folterbauer, C.; Kurz, A.; Fiechtner, B.; Möst, M.; Salvenmoser, W.; Sassone- Corsi, P.; Pelster, B. (2013): Linking oxygen to time: The bidirectional interaction between the hypoxic signaling pathway and the circadian clock.
    In: CHRONOBIOLOGY INTERNATIONAL 30/4, pp. 510 - 529. (DOI) (Web link)

  • Egg, M.; Tischler, A.; Schwerte, T.; Sandbichler, A.; Folterbauer, C.; Pelster, B. (2012): Endurance exercise modifies the circadian clock in zebrafish (Danio rerio) temperature independently.
    In: Acta Physiologica 205/1, pp. 167 - 176. (DOI) (Web link)

  • Kopp, R.; Köblitz, L.; Egg, M.; Pelster, B. (2011): HIF - signaling and overall gene expression changes during hypoxia and prolonged exercise differ considerably.
    In: PHYSIOLOGICAL GENOMICS 43/9, pp. 506 - 516. (DOI)

  • Palacio, Oscar; Pagani, Ayelen; Pérez-Rafael, Silvia; Egg, Margit; Höckner, Martina; Brandstätter, Anita; Capdevila, Mercé; Atrian, Silvia; Dallinger, Reinhard (2011): Shaping mechanisms of metal specificity in a family of metazoan metallothioneins: evolutionary differentiation of mollusc metallothioneins.
    In: BMC Biology 9/4, pp. 1 - 20. (DOI)

  • Sandbichler, A.M.; Egg, M.; Schwerte, T.; Pelster, B. (2011): Claudin 28b and F-actin are involved in rainbow trout gill pavement cell tight junction remodeling under osmotic stress.
    In: Journal of Experimental Biology 214/9, pp. 1473 - 1487. (DOI)

  • Kopp, R.; Schwerte, T.; Egg, M.; Sandbichler, A.M.; Egger, B.; Pelster, B. (2010): Chronic reduction in cardiac output induces hypoxic signaling in larval zebrafish even at a time when convective oxygen transport is not required.
    In: PHYSIOLOGICAL GENOMICS 42a, pp. 8 - 23. (DOI)

  • Egg, M.; Höckner, M.; Brandstätter, A.; Schuler, D.; Dallinger, R. (2009): Structural and bioinformatic analysis of the Roman snail Cd-Metallothionein gene uncovers molecular adaptation towards plasticity in coping with multifarious environmental stress.
    In: Molecular Ecology 18/11, pp. 2426 - 2443.

  • Zoller, H.; Egg, M.; Graziadei, I.; Creus, M.; Janecke, A. R.; Löffler-Ragg, J.; Vogel, W. (2007): CFTR gene mutations in pancreatitis: Frequency and clinical manifestations in an Austrian patient cohort.
    In: Wiener Klinische Wochenschrift 119/17-18, pp. 527 - 533.

  • DALLINGER, R.; LAGG, B.; EGG, M.; SCHIPFLINGER, R.; CHABICOVSKY, M. (2004): Cd accumulation and Cd-Metallothionein as a biomarker in Cepaea hortensis (Helicidae, Pulmonata) from laboratory exposure and metal-polluted habitats.
    In: ECOTOXICOLOGY 13, pp. 757 - 772.

Journal Article (Proceedings Paper)
  • Pelster, B.; Egg, M. (2007): The influence of hypoxia on developing zebrafish.
    In: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY 148/Supplement 1, pp. 118 - 119. (DOI)

Journal Article (Meeting-Abstract)
  • Dallinger, Reinhard; Höckner, Martina; Egg, Margit; Palacios, Oscar; Capdevila, Mercé; Pagani, Ayelen; Atrian, Silvia (2010): Structural and functional differentiation of gastropod metallothioneins has been achieved by evolution of metal-specific isoform families.
    In: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY 157/Supplement 1, p. S35. (DOI)

  • Dallinger, Reinhard; Egg, Margit; Höckner, Martina; Schuler, Dietmar; Atrian, Silvia; Capdevila, Mercé (2009): Molecular adaptation to cope with environmental stress of terrestrial gastropod Cadmium-metallothioneins: High metal specificity at the protein level combines with multifunctionality of genes.
    In: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY 154A/1 (Suppl.), p. S33.

  • Egg, M.; Pelster, B. (2009): Hypoxia modifies the expression of clock genes in zebrafish larvae.
    In: FASEB JOURNAL 23, p. 779.3.

  • Atrian, Silvia; Pagani, Ayleen; Capdevila, Mercé; Palacios, Oscar; Egg, Margit; Höckner, Martina; Dallinger, Reinhard (2008): Perfect adaptation to cope with metal stress and trace element homeostasis: Metal specificity is a fundamental structural feature of snail metallothioneins.
    In: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY 151A/1 (Suppl.), p. 520.

  • Dallinger, Reinhard; Egg, Margit; Höckner, Martina; Schuler, Dietmar (2008): Understanding the functioning of a Gene: Ability of the Cd-Metallothionein of Roman snail (Helix pomatia L.) to cope with Multiple Environmental Stress.
    In: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY 151A/1 (Suppl.), p. 520.

  • Egg, M.; Moest, M.; Pelster, B. (2008): Hypoxia - a zeitgeber of the circadian clock?
    In: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY 150/3 (Supplement 1), pp. 151 - 152.

  • Pelster, B.; Kopp, R.; Egg, M. (2008): Hypoxia during development of the zebrafish – signalling and effects.
    In: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY 151/1 (Supplement 1), p. 1.

  • Sandbichler, AM.; Egg, M.; Pelster, G.B. (2005): Osmolarity-induced changes in trout gill epithelial cells on permeable supports.
    In: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY 141/Supplement 1, p. 197.



Lectures

Presentations at Conferences, Symposia, etc.

Conference Lecture (Invited Lecture)
  • Lecturer(s): Egg, M. Co-author(s): Sandbichler, A.: Linking oxygen to time: The impact of oxygen availability on the circadian clock in zebrafish.
    The 29th Conference of the International Society for Chronobiology (ISC), Suzhou, 2016-10-26. (Web link)

  • Lecturer(s): Pelster, B. Co-author(s): Egg, M.: Hif-signaling is interconnected with the circadian clock.
    Frontiers in Tropical Aquatic Physiology, Manaus, 2013-05-10.

  • Lecturer(s): DALLINGER, R. Co-author(s): EGG, M.; ATRIAN, S.: Molecular adaptation at different levels of biological organization: How terrestrial pulmonates solve their metal problem.
    24th Annual Meeting of the European Society for Comparative Physiology and Biochemistry (ESCPB), Antwerp, 2006-09-19.

Conference Lecture (Upon Registration)
  • Lecturer(s): Tischler, A. Co-author(s): Egg, M.; Pelster, B.; Füreder, L.: Heat shock protein expression in Diamesa latitarsis (Diptera, Chironomidae) under natural conditions.
    9th Symposium for European Freshwater Sciences, Genf, 2015-07-05. (Web link)

  • Lecturer(s): Egg, M. Co-author(s): Schwerte, T.; Sandbichler, A.M.; Pelster, B.: Linking time to oxygen: The cross-talk between the Circadian Clock and the Hypoxic Signaling Pathway.
    Society for Experimental Biology Annual Meeting 2014, Manchester, 2014-07-03. (Web link)

  • Lecturer(s): Sandbichler, A.M. Co-author(s): Nagy, J.; Egg, M.: Differential hypoxic response of the circadian clock: attenuation versus phase shift.
    Society for Experimental Biology Annual Meeting 2014, Manchester, 2014-07-03. (Web link)

  • Lecturer(s): Pelster, B. Co-author(s): Egg, M.: Hypoxia Induced Hif Signaling in Zebrafish Larvae.
    10th International Congress on the Biology of Fish, Madison, 2012-07-18. (Web link)

  • Lecturer(s): Pelster, B. Co-author(s): Egg, M.: Prolonged swimming activity modifies the expression of clock genes in zebrafish.
    10th International Congress on the Biology of Fish, Madison, 2012-07-18. (Web link)

  • Lecturer(s): Atrian, Silvia Co-author(s): Pagani, Ayleen; Capdevila, Mercé; Palacios, Oscar; Egg, Margit; Höckner, Margit; Dallinger, Reinhard: Evolutionary diversification of snail metallothioneins is driven by extreme metal specificity of isoforms combined with cell-specific expression.
    5th Annual Meeting of the Center of Molecular Biosciences (CMBI), Igls, 2008-09-26.

  • Lecturer(s): Egg, M. Co-author(s): Möst, M.; Pelster, B.: Hypoxia- a zeitgeber of the circadian clock?
    Society for Experimental Biology Annual Main Meeting, Marseille, 2008-07-06.

  • Lecturer(s): DALLINGER, R.; EGG, M.; CAPDEVILA, M.; ATRIAN, S: How to cope with metal stress in a changeful environment - Lessons learned from terrestrial pulmonate mulluscs.
    3rd Annual Meeting of the Center of Molecular Biosciences (CMBI), Vill, 2006-09-30.

  • Lecturer(s): Egg, M.: The Cd- Metallothionein gene of Helix pomatia: An evolutionary key to the understanding of complex stress response?
    2nd Annual Meeting of the Center for Molecular Biosciences (CMBI), Vill, 2005-09-30.

  • Lecturer(s): EGG, M.; HÖCKNER, M.; DALLINGER, R.: Vom Protein zum Gen – das Biomarker-Potential der Metallothionein-Antwort in der Weinbergschnecke und dessen ökologische Relevanz.
    10. SETAC GLB – Meeting, Basel, 2005-09-29.

Poster Presentation
  • Lecturer(s): Sandbichler, A.M. Co-author(s): Scheidnagl, S.; Selcuk, D.; Rainer, D.; Pelster, B.; Egg, M.: Mitochondrial plasticity in zebrafish fibroblasts exposed to hypoxia.
    Mitochondrial Trafficking and Function in Health and Disease, Boston, 2012-06-25. (Web link)

  • Lecturer(s): Dallinger, Reinhard; Höckner, Martina; Schuler, Dietmar; Egg, Margit; Atrian, Silvia; Capdevial, Mercé: Understanding multifunctionality of terrestrial gastropod Cadmium-Metallothioneins: High metal specificity of expressed protein is complemented by response plasticity at the gene level.
    1st Life Science Meeting, Igls, 2009-09-18.

  • Lecturer(s): Egg, M.; Pelster, B.: Hypoxia modifies the expression of clock genes in zebrafish larvae.
    Experimental Biology 2009, New Orleans, 2009-04-18.

  • Lecturer(s): Egg, M.; Pelster, B.: Crosstalk between the circadian clock and the hypoxic pathway in zebrafish (Danio rerio).
    3rd European Science Foundation Functional Genomics Conference 2008, Innsbruck, 2008-10-01.

  • Lecturer(s): Egg, Margit; Höckner, Martina; Brandstätter, Anita; Schuler, Dietmar; Dallinger, Reinhard: Plasticity in coping with multifarious Environmental Stress by Roman Snail Populations is linked to the structure and responsiveness of the Cd-Metallothionein Gene: a bioinformatic analysis.
    5th Annual Meeting of the Center of Molecular Biosciences (CMBI), Igls, 2008-09-26.

  • Lecturer(s): ATRIAN, S.; CAPDEVILA, M.; PAGANI, A.; PALACIOS, O.; EGG, M.; DALLINGER, R.: Functional differentiation of metal-specific snail metallothionein (MT) native isoforms is confirmed by characterization of recombinantly synthesized metal-MT complexes.
    4th Annual Meeting of the Center of Molecular Biosciences (CMBI), Igls, 2007-09-29.

  • Lecturer(s): DALLINGER, R.; EGG, M.; HÖCKNER, M.; SCHULER, D.: The Cd-Metallothionein of the Roman snail Helix pomatia: Specific adaptation of a gene to cope with multifarious environmental stress.
    4th Annual Meeting of the Center of Molecular Biosciences (CMBI), Igls, 2007-09-29.

  • Lecturer(s): SCHULER, D.; EGG, M.; HISPARD, F.; de VAUFLEURY, A.; DALLINGER, R.: Characterization of Metallothionein isoforms in Cornu aspersum (Pulmonata) by a combined molecular and biochemical approach.
    3rd Annual Meeting of the Center of Molecular Biosciences (CMBI), Vill, 2006-09-30.

  • Lecturer(s): Sandbichler, A.; Egg, M.; Pelster, B.: The influence of apical hypoosmotic stress on the expression of cytoskeletal components in trout gill epithelial cells.
    7th International Congress on the Biology of Fish (ICBF VII), St. John’s, 2006-07-18.

  • Lecturer(s): Dallinger, R.; Egg, M.; Chabicovsky, M.: The dual metallothionein system of Helix pomatia: towards its application in environmental toxicology.
    2nd Annual Meeting of the Center for Molecular Biosciences (CMBI), Vill, 2005-09-30.

  • Lecturer(s): EGG, M.; HÖCKNER, M.; DALLINGER, R.: The Cd-metallothionein gene of Helix pomatia: An evolutionary key to the understanding of complex stress response?
    2nd Annual Meeting of the Center for Molecular Biosciences (CMBI), Vill, 2005-09-30.

  • Lecturer(s): Prem, C.; Egg, M.; Hauffe, H.; Schwerte, T.; Pelster, B.: Influence of swim training on erythropoietin expression in zebrafish larvae (Danio rerio).
    2nd Annual Meeting of the Center for Molecular Biosciences (CMBI), Vill, 2005-09-30.

  • Lecturer(s): Sandbichler, A.; Egg, M.; Pelster, B.: Transcriptional response to apical osmotic stress in trout gill epithelial cells.
    2nd Annual Meeting of the Center for Molecular Biosciences (CMBI), Vill, 2005-09-30.

  • Lecturer(s): Dallinger, R.; Egg, M.: Die Bedeutung ökotoxikologischer Grundlagenforschung für die Einschätzung und prognostische Beurteilung der Zustands- Entwicklung alpiner Lebensräume.
    Die Alpen im Jahr 2020. Erkenntnisse, Thesen, Szenarien, Fragen, Obergurgl, 2005-09-20.

  • Lecturer(s): Sandbichler, A.; Egg, M.; Pelster, B.: Transcriptional response to apical osmotic stress in trout gill epithelial cells.
    SEB Annual Main Meeting 2005, Barcelona, 2005-07-11.

  • Lecturer(s): Egg, Margit; Höckner, Martina; Dallinger, Reinhard: Analysis of gene control regions of Cd- and Cu-metallothionein isoforms from Helix pomatia reveal remarkable structural features suggesting complex regulatory functions.
    1st Annual Meeting of the Center for Molecular Biosciences (CMBI), Vill, 2004-10-01.

Guest Lectures

Guest Lecture
  • Lecturer(s): Dallinger, R.; Chabicovsky, M.; Egg, M.: Cu regulation by subcellular speciation: Metal compartmentalization and the role of Cu- Metallothionein in a terrestrial snail.
    Universität Zürich, Zürich, 2005-12-01.

  • Lecturer(s): Egg, M.; Höckner, M.; Dallinger, R.: The Cd-Metallothionein gene of Helix pomatia: a prime example for gene functioning?
    Universität Zürich, Zürich, 2005-12-01.



Projects, scholarships and other third-party funds

Research Projects

  • Egg, Margit; Paulitsch, Monika: The impact of hypoxia on metabolic oscillators.
    Tiroler Wissenschaftsförderung (TWF), Nachwuchsförderung
    2017-01-01 - 2017-12-31

  • Egg, Margit: NMR, Hif und Clock. Auswirkung der Kernspinresonanz Therapie NMR auf die zirkadiane Uhr und den Hypoxie – Signalweg.
    MedTec Medizintechnik GmbH
    2015-05-15 - 2018-05-14

  • Egg, Margit: Auswirkungen von Therapeutic-NMR (MBST®-Nuclear Magnetic Resonanz) auf die circadiane Uhr und den Hypoxie-Signalweg in Zebrafisch-Zellen.
    MedTec Medizintechnik GmbH
    2014-02-01 - 2014-07-31

  • Dallinger, Reinhard; Egg, Margit: Metal-specific adaptation of metallothionein isoforms and their genes in Helix pomatia: Metal preference studies by recombinant expression in vitro and construction of transcriptional reporter genes transfected in Biomphalaria glabrata embryonic cells.
    Fonds zur Förderung der wissenschaftlichen Forschung (FWF), Einzelprojekt
    2007-04-01 - 2010-12-31



Professional Posts and Affiliations

  • Egg, M.: Mitglied des Fakultätsrates.
    Leopold-Franzens-Universität Innsbruck - Fakultät für Biologie, Innsbruck, 2008-01-01 on-going.

  • Egg, M.: Mitglied im Berufungsverfahren für die Professur "Zoologie".
    Leopold-Franzens-Universität Innsbruck, Innsbruck, 2007-09-01 - 2008-06-30.



Academic Activities and Achievements

Supervision of Student Theses

  • Egg, Margit: Kurz, Antje: Impact of Clock3 on the Hypoxic Response of Transgenic Zebrafish Cell Lines (2010).

  • Egg, Margit: Tischler, Astrid: Exercise: A Zeitgeber for the Circadian Clock in Zebrafish (Damio rerio) (2010).