Course Listing and Outline
Lectures
Number: 712001| Instructor: Kwiatkowski
A) Chemical Components of the Cell
1. Subject and Unifying Principles of Biochemistry
2. Water, Acids and Bases
3. Amino Acids, Peptides, and Proteins
4. Structure and Function of Proteins
5. Structure and Function of Enzymes
6. Vitamines and Coenzymes
7. Lipids
8. Carbohydrates
9. Nucleic Acids
B) Metabolism
10. Principal Metabolic Strategies
11. Glycolysis
12. Citric Acid Cycle
13. Oxidative Phosphorylation
14. Pentose Phosphate Pathway
15. Gluconeogenesis
16. Glycogen Metabolism
17. Fatty Acid Metabolism
18. Amino Acid Degradation
Number: 712002| Instructor: Kwiatkowski
B) Metabolism (continued)
19. Photosynthesis
20. Biosynthesis of Complex Lipids and Steroids
21. Biosynthesis of Amino Acids
22. Biosynthesis and Degradation of Heme
23. Biosynthesis and Degradation of Nucleotides
24. Coordination of Metabolism
C) Biochemistry and Molecular Genetics
25. Structure and Replication of DNA
26. Transcription and RNA Processing
27. Protein Synthesis
28. Regulation of Gene Expression in Prokaryotes
29. Eukaryotic Chromosomes and Gene Expression
30. Gene Technology
D) Biochemical Basis of Complex Biological Processes
31. Molecular Immunology
32. Muscle Contraction and Motility
33. Hormone Function
34. Signal Transduction
35. Membrane Transport
Number: 712005| Instructor: Hartl, Kwiatkowski
1. Preparation and Electrophoresis of Nucleic Acids
2. Hybridization and Detection of Nucleic Acids
3. Gene Libraries and Genome Analysis
4. Monitoring of Differential Gene Activity
5. Analysis of Promoters and of Chromatin
6. DNA Sequencing and Bioinformatic Sequence Analysis
7. Preparation and Analytics of Recombinant Proteins
8. Mass Spectrometry of Proteins
9. Spectroscopical Methods
10. X-ray Structure Analysis of DNA and Proteins
11. Nuclear Magnetic Resonnance Spectroscopy of Proteins
12. Electron Microscopic Investigations
13. Analysis of Protein-Nucleic Acid Interactions
14. Analysis of Protein-Protein Interactions
15. Immunological Techniques for Protein Detection
16. Analysis of Cellular Proteins
17. Cell Biological Methods
18. Exploring Mechanisms of Signal Transduction
19. Gene Modification and Reverse Genetics
20. Specific Gene Inactivation
Number: 740050| Instructor: Hartl
A) Molecular Design of Life
1. Biochemistry: an Overview
2. Protein Structure and Function
3. Protein and Proteome Analysis
4. Nucleic Acids and Genetic Flow
5. Evolution and Bioinformatics
6. Enzymes and Inhibitors
7. Vitamins and Coenzymes
8. Carbohydrates and Glycosylation
9. Lipids and Cell Membranes
10. Metabolism: Concepts and Design
B) Generation and Storage of Energy
11. Glycolysis and Gluconeogenesis
12. Citric acid Cycle and Respiration
13. Photosynthesis
14. Pentose Phosphate Pathway
15. Glycogen Metabolism
16. Fatty acid Metabolism
17. Amino Acid Metabolism
18. Nucleotide Metabolism
19. Synthesis of Complex Lipids
20. Synthesis of Steroids
C) Molecular Biology and Complex Systems
21. DNA Replication, Recombination, Repair
22. RNA Synthesis and Processing
23. Protein Biosynthesis
24. Gene Expression Control
25. Gene and Genome Analysis
26. Signal Transduction
27. Sensory Systems
28. Immune System
29. Molecular Motors
30. Drug Development
Number: 712004| Instructor: Hartl
An Advanced Treatment of:
1. Amino Acid Metabolism
2. Regulation of Cholesterol Metabolism
3. Steroid Hormones / Isoprenoid Compounds
4. Structure and Chemical Attributes of DNA
5. Gene-Protein Relation
6. Protein Targeting
7. Mechanisms of Gene Regulation
8. Signal Transduction
Number: 712901| Instructor: Ramos Pittol
An Advanced Treatment of:
1. Nucleic Acid Hybridization
2. Gene Libraries and Gene Mapping
3. Differential Gene Expression
4. Promoter Analysis
5. Chromatin Analysis
6. Bioinformatic Sequence Analysis
Number: 712902| Instructor: N.N.
An Advanced Treatment of:
1. Recombinant Proteins and Synthetic Peptides
2. Molecular Separation Techniques
3. Mass Spectrometry of Proteins
4. Spectroscopic Methods
5. X-ray Analysis of DNA and Proteins
6. Nuclear Magnetic Resonance (NMR) Spectroscopy of Proteins
Number: 712903| Instructor: N.N.
An Advanced Treatment of:
1. Preparation of Nucleic Acids
2. Radioactive Labeling of DNA
3. Analysis of Protein-Protein Interactions
4. Analysis of Protein-DNA Interactions
5. Protein Analysis using Immunological Techniques
6. Electron Microscopy
Number: 712904| Instructor: N.N.
An Advanced Treatment of:
1. Gene Modifications
2. Targeted Gene Silencing
3. Cell Biological Methods
4. Cell Systems
5. Molecular Mechanisms of Cell Proliferation
6. Drug Development
Number: 712905, 712906| Insturctor: Hartl, Ramos Pittol
Presentation and discussion of current research results and biochemical topics.
Lab Courses
Number: 712007| Instructor: Hatzmann, Ramos Pittol, Schäfer, Sprenger, Timpen
Experiment 1: Preparation of high molecular weight DNA
Methodology: Isolation of eukaryotic genomic DNA, photometric analysis of nucleic acids
Experiment 2: Molecular cloning and sequence analysis of DNA
Methodology: Isolation of plasmid DNA, restriction enzyme digestion of DNA and agarose gel electrophoresis, cycle sequencing of DNA
Experiment 3: Synthesis and modification of DNA
Methodology: Polymerase chain reaction (PCR), nucleic acid hybridization, radioactive end-labeling of DNA
Experiment 4: DNA-protein interaction
Methodology: Electrophoretic mobility shift assay (EMSA), native polyacrylamide gel electrophoresis, phosphor imaging
Experiment 5: Protein-protein interaction
Methodology: Computerized protein sequence analysis, sequence alignments, protein fragment complementation assay, bioluminescence readout
Experiment 6: RNA preparation
Methodology: Isolation of total RNA from eukaryotic cells, preparative ultracentrifugation
Experiment 7: Differential gene expression analysis
Methodology: Reverse transcription of mRNA, PCR amplification of cDNA, agarose gel electrophoresis, real-time PCR
Experiment 8: Protein expression and purification
Methodology: Recombinant protein expression, affinity chromatography, fast protein liquid chromatography (FPLC)
Experiment 9: Protein analysis
Methodology: SDS polyacrylamide gel electrophoresis, protein staining, molecular weight determination
Experiment 10: Protein identification
Methodology: Immunoblotting of proteins, detection by reporter enzymes coupled to antibodies
Number: 740550| Instructor: Hatzmann, Ramos Pittol, Schäfer, Sprenger, Timpen
Experiment 1: Preparation of high molecular weight DNA
Methodology: Isolation of eukaryotic genomic DNA, photometric analysis of nucleic acids
Experiment 2: Molecular cloning and sequence analysis of DNA
Methodology: Isolation of plasmid DNA, restriction enzyme digestion of DNA and agarose gel electrophoresis, cycle sequencing of DNA
Experiment 3: Differential gene expression analysis
Methodology: Reverse transcription of mRNA, PCR amplification of cDNA, agarose gel electrophoresis, real-time PCR
Experiment 4: Protein expression and purification
Methodology: Recombinant protein expression, affinity chromatography, fast protein liquid chromatography (FPLC)
Experiment 5: Protein analysis
Methodology: SDS polyacrylamide gel electrophoresis, protein staining, molecular weight determination
Number: 712012, 712013| Instructor:Hartl, Hatzmann, Ramos Pittol, Schäfer, Sprenger, Timpen
Students taking this course will participate in current research projects and will receive hands-on training in modern techniques in the fields of biochemistry, molecular biology, and gene technology. Students taking the lab course have also to enroll in the parallel introductory course (712015). Both courses are offered year-round and registration is possible any time. Prospective participants must have completed the lectures (712003 and 712004) and laboratory course in basic biochemistry (712007).
Number: 712014, 712015| Instructor: Hartl, Hatzmann, Ramos Pittol
Participation in challenging research projects at an advanced stage.