Adolf Sandbichler

Research assistant

Adolf Sandbichler


Address:
Department of Zoology
University of Innsbruck

Technikerstr. 25
6020 Innsbruck
Austria

Room: 6/20
Phone: +43 512 507-51868
e-mail: adolf.sandbichler@uibk.ac.at

Twitter: ASandbichler

Current research

In my research I am interested in the influence of environmental stress such as osmotic changes and low oxygen availability on the acclimation and homeostasis of fish primary cell cultures and cell lines. I study the cell physiological responses with focus on live cell imaging and measurement. One aim is the exact characterization of reactive oxygen species (ROS) emerging in response to a hypoxic exposure and their interaction with cell metabolism. Here my work also intersects with research on circadian rhythms since ROS are suspected as being a linker between the different signalling pathways.

Furthermore I manage matters concerning method development and laser scanning microscopy at the institute in teaching and research.

For more details see:

 Here you can find more about me in a series called "350 Years University of Innsbruck, Together we are University" (September 2019)

Student courses

I am a teacher in the master's programme "Molecular Cell and Developmental Biology" (MolZEB).

  • Winter semester (October):
    Elective module 9: Zellbiologie II: Zellhomöostase (7,5 ECTS-AP, 5 SSt)
    719515 Zellhomöostase (SE)
    719516 Zellhomöostase (UE)
  • Summer semester (March):
    Elective module 7: Live Cell Imaging (7,5 ECTS-AP, 5 SSt)
    719301 Live Cell Imaging (VO)
    719302 Live Cell Imaging (UE)


Administrative work

Since 2006 I am trusted representative for disabled scientists at the University of Innsbruck workers council.

 

Curriculum vitae

2003 MSc., with distinction. Thesis supervised by Bernd Pelster: Acid-base regulation in isolated gill cells of the goldfish (Carassius auratus). Institute of Zoology, University of Innsbruck
2004 Research assistant in training, Institute of Zoology, University of Innsbruck
2009 PhD., Dissertation thesis supervised by Bernd Pelster: Transcriptional and Morphological Responses to Osmotic Stress in Trout Gill Cell Cultures: Dynamics of the Tight Junction Protein Claudin 28b and Cortical F-actin in Epithelial Adaptation. Institute of Zoology, University of Innsbruck
since 2009 Research assistant, Institute of Zoology, University of Innsbruck
2010 Attendance at the 2nd IPMB ‐Workshop on Real‐time Monitoring of Cellular
Metabolism using Cell Biosensor Chips. Institute of Pharmacy and Molecular
Biotechnology, Ruprecht‐Karls‐University Heidelberg, Germany
2011 Attendance at the "International Advanced Confocal Microscopy Course" at Leica Microsystems, Mannheim, Germany and the "Advanced Techniques in Laser Scanning Microscopy Course" at Carl Zeiss Microimaging, Jena, Germany
2012 Start of the Leica SP5 II TCS laser scanning microscope at the Institute of Zoology
In-depth training on the MoFlo Astrios (FACS) by Beckman Coulter at the Institute of Ecology
2014 In-depth training on the Leica SP8 gatedSTED Superresolution microscope at the CCB Innsbruck Universities
2014 Introduction of the CellASIC ONIX (Merck Millipore) live cell imaging platform at the Institute of Zoology
2015 Introduction of the Seahorse XFp (Agilent Technologies) cellphysiometer at the Institute of Zoology
2017 Establishment of a new circadian lighting system in cooperation with LDDE GmbH, Vienna
2018 Attendance at the "2018 Zurich Imaging and Data Analysis School", ZIDAS, ETH Zurich
2019 Introduction of a new LC-MS unit (Waters Aquity Arc, QDa) at the Institute of Zoology

 

Projects

  • Reactive Oxygen Species and their role in the interaction between Circadian Clock and Hypoxic Signaling Pathway, Forschungsförderungsmittel der Nachwuchsförderung 2015 der Universität Innsbruck
  • Anpassung von Kiemenepithelzellen an osmotische Veränderungen, Tiroler Wissenschaftsfonds (TWF), 2005


Publications

Publications

Contributions to Books / Journals

Journal Article (Original Paper)
  • Oliva, R.; Jansen, B.; Benscheidt, F.; Sandbichler, A.M.; Egg, M. (2019): Nuclear magnetic resonance affects the circadian clock and hypoxia-inducible factor isoforms in zebrafish.
    In: Biological Rhythm Research 50/5, pp. 739 - 757. (DOI) (Web link)

  • Sandbichler, A.M.:; Jansen, B.; Peer, B.A..; Paulitsch, M.; Pelster, B.; Egg, M. (2018): Metabolic Plasticity Enables Circadian Adaptation to Acute Hypoxia in Zebrafish Cells.
    In: CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 46/3, pp. 1159 - 1174. (URN) (DOI)

  • Egg, M.; Paulitsch, M.; Ennemoser, Y.; Wüstenhagen, A.; Schwerte, T.; Sandbichler, A. M.; Fiechtner, B.; Köblitz, L.; Prem, C.; Pelster, B. (2014): Chronodisruption increases cardiovascular risk in zebrafish via reduced clearance of senescent erythrocytes.
    In: CHRONOBIOLOGY INTERNATIONAL 31/5, pp. 680 - 689. (DOI)

  • Leis, S.; Dresch, P.; Peintner, U.; Fliegerova, K.; Sandbichler, A.M.; Insam, H.; Podmirseg, Sabine Marie (2014): Finding a robust strain for biomethanation: anaerobic fungi (Neocallimastigomycota) from the Alpine ibex (Capra ibex) and their associated methanogens.
    In: ANAEROBE 29, pp. 34 - 43. (DOI) (Web link)

  • Sandbichler, A.M.; Aschberger, T.; Pelster, B. (2013): A Method to Evaluate the Efficiency of Transfection Reagents in an Adherent Zebrafish Cell Line.
    In: BioResearch Open Access 2/1, pp. 20 - 27. (DOI) (Web link)

  • Egg, M.; Tischler, A.; Schwerte, T.; Sandbichler, A.; Folterbauer, C.; Pelster, B. (2012): Endurance exercise modifies the circadian clock in zebrafish (Danio rerio) temperature independently.
    In: Acta Physiologica 205/1, pp. 167 - 176. (DOI) (Web link)

  • Sandbichler, A.M.; Egg, M.; Schwerte, T.; Pelster, B. (2011): Claudin 28b and F-actin are involved in rainbow trout gill pavement cell tight junction remodeling under osmotic stress.
    In: Journal of Experimental Biology 214/9, pp. 1473 - 1487. (DOI)

  • Sandbichler, A.M.; Farkas, J.; Salvenmoser, W.; Pelster, B. (2011): Cortisol affects tight junction morphology between pavement cells of rainbow trout gills in single-seeded insert culture.
    In: Journal of Comparative Physiology B: Biochemical, Systemic, and environmental physiology 181/8, pp. 1023 - 1034. (DOI)

  • Kopp, R.; Schwerte, T.; Egg, M.; Sandbichler, A.M.; Egger, B.; Pelster, B. (2010): Chronic reduction in cardiac output induces hypoxic signaling in larval zebrafish even at a time when convective oxygen transport is not required.
    In: PHYSIOLOGICAL GENOMICS 42a, pp. 8 - 23. (DOI)

  • Martien, R.; Loretz, B.; Sandbichler, A.M.; Bernkop-Schnürch, A. (2008): Thiolated chitosan nanoparticles: transfection study in the Caco-2 differentiated cell culture.
    In: NANOTECHNOLOGY 19, pp. 1 - 9.

  • SANDBICHLER, AM.; PELSTER, B. (2004): Acid-Base Regulation in Isolated Gill Cells of the Goldfish (Carassius auratus).
    In: Journal of Comparative Physiology B: Biochemical, Systemic, and environmental physiology 174/8, pp. 601 - 610.

Journal Article (Review)
  • Sandbichler, A.M.; Höckner, M. (2016): Cadmium Protection Strategies-A Hidden Trade-Off?
    In: International Journal Of Molecular Sciences 17/1, No. 139. (DOI) (Web link)

Journal Article (Meeting-Abstract)
  • Sandbichler, A. M.; Schwerte, T.; Pelster, B. (2009): Cortisol-dependent remodelling of tight junction claudin 28b and cortical F-actin in cultured gill epithelia of rainbow trout.
    In: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY 154/1 (Supplement 1), p. S29.

  • Aigner, M.; Botta, M.; Sandbichler, A.; Salvenmoser, W.; Egger, B.; Rieger, R.; Gschwentner, R. (2005): Development of a basal medium for primary cell culture of marine flatworms.
    In: Berichte des Naturwissenschaftlich-Medizinischen Vereins in Innsbruck 16, p. 20.

  • Sandbichler, AM.; Egg, M.; Pelster, G.B. (2005): Osmolarity-induced changes in trout gill epithelial cells on permeable supports.
    In: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY 141/Supplement 1, p. 197.

Other Publications

Electronic Publication, Scientific
  • Badstöber, J.; Gachon, C.M.M.; Sandbichler, A.; Neuhauser, S. (2019): Biotrophic interactions disentangled: In-situ localisation of mRNAs to decipher plant and algal pathogen – host interactions at single cell level. (DOI) (Web link)



Lectures

Presentations at Conferences, Symposia, etc.

Conference Lecture (Invited Plenary / Keynote Lecture)
  • Lecturer(s): Neuhauser, S. Co-author(s): Badstöber, J.; Loacker, A.; Ciaghi, S.; Harikrishnan, S.; Sandbichler, A. M.; Kirchmair, M.; Gachon, MM C.: Biotrophic interactions untangled: In-situ localization of mRNAs is a powerful tool to study pathogen – host interactions at the single cell level.
    SAMS-Algal Research for Policy-Making and Biotech, Oban, 2018-05-15. (Web link)

Conference Lecture (Invited Lecture)
  • Lecturer(s): Neuhauser, Sigrid Co-author(s): Kirchmair, Martin; Ciaghi, Stefan; Badstöber, Julia; Schwelm, Arne; Ludwig Müller, Jutta; Sandbichler, Adolf M.; Gachon, Claire: Understanding phytomyxid-host interactions by combining transcriptomics with in-situ transcript visualisation.
    38th Annual Meeting of the German Society for Protozoology, Wien, 2019-02-21.

  • Lecturer(s): Egg, M. Co-author(s): Sandbichler, A.: Linking oxygen to time: The impact of oxygen availability on the circadian clock in zebrafish.
    The 29th Conference of the International Society for Chronobiology (ISC), Suzhou, 2016-10-26. (Web link)

Conference Lecture (Upon Registration)
  • Lecturer(s): Egg, M. Co-author(s): Schwerte, T.; Sandbichler, A.M.; Pelster, B.: Linking time to oxygen: The cross-talk between the Circadian Clock and the Hypoxic Signaling Pathway.
    Society for Experimental Biology Annual Meeting 2014, Manchester, 2014-07-03. (Web link)

  • Lecturer(s): Sandbichler, A.M. Co-author(s): Nagy, J.; Egg, M.: Differential hypoxic response of the circadian clock: attenuation versus phase shift.
    Society for Experimental Biology Annual Meeting 2014, Manchester, 2014-07-03. (Web link)

  • Lecturer(s): Leis, S. Co-author(s): Dresch, P.; Peintner, U.; Fliegerová, K.; Sandbichler, A.M.; Insam, H.; Podmirseg, Sabine Marie: Isolation and identification of anaerobic fungi (Neocallimastigomycota) from an alpine herbivore, the capricorn (Capra ibex).
    8th International Symposium on Anaerobic Microbiology (ISAM8), Innsbruck, 2013-06-13.

  • Lecturer(s): Sandbichler, A.: Osmolarity-induced changes in trout gill epithelial cells on permeable supports.
    SEB Annual Main Meeting 2005, Barcelona, 2005-07-11.

Lecture at Expert Conference
  • Lecturer(s): Egg, M. Co-author(s): Oliva, R.; Benscheidt, F.; Sandbichler, A.M.: Wirkung der MBST Kernspinresonanztherapie (MBST NMR) auf die Innere Uhr und den Sauerstoff-Signalweg.
    Anwendertagung / Conference: Therapie par resonance magnetique nucleaire, St. Chamond, Lyon, 2017-10-19.

Poster Presentation
  • Lecturer(s): Badstöber, J; Gachon, CMM; Sandbichler, A; Neuhauser, S: Biotrophic interactions disentangled: In-situ localisation of mRNAs to decipher plant and algal pathogen - host interactions at single cell level.
    Kick-off Meeting Life & Health Science Cluster Tirol, Innsbruck, 2018-12-04. (Web link)

  • Lecturer(s): Dikova, V. Co-author(s): Vorhauser, J.; Pelster, B.; Sandbichler, A.M.: Hydrogen Peroxide under Hypoxia/Reoxygenation in Zebrafish Fibroblasts.
    7th Annual CMBI Meeting Innsbruck, Vill, 2018-09-20. (Web link)

  • Lecturer(s): Kaehler, Kathrin Co-author(s): Seitter, Hartwig; Sandbichler, Adolf; Obermair, Gerald; Stefanova, Nadia; Koschak, Alexandra: The Neuroretina in Multiple System Atrophy: Morphological Implications of Plp-α-Syn Mice.
    47th Annual Meeting of the Society for Neuroscience, Washington D.C., 2017-11-14. (Web link)

  • Lecturer(s): Kaehler, Kathrin Co-author(s): Seitter, Hartwig; Sandbichler, Adolf; Obermair, Gerald; Stefanova, Nadia; Koschak, Alexandra: The neuroretina in multiple system atrophy: morphological implications of Plp-α-Syn mice.
    European Retina Meeting 2017 (ERM), Paris, 2017-10-06. (Web link)

  • Lecturer(s): Kaehler, Kathrin Co-author(s): Seitter, Hartwig; Sandbichler, Adolf; Obermair, Gerald; Stefanova, Nadia; Koschak, Alexandra: Does the Pathology of Multiple System Atrophy manifest in the Neuroretina?
    23nd Scientific Symposium of the Austrian Pharmacological Society (APHAR), Innsbruck, 2017-09-29. (Web link)

  • Lecturer(s): Kaehler, Kathrin Co-author(s): Seitter, Hartwig; Sandbichler, Adolf; Obermair, Gerald; Stefanova, Nadia; Koschak, Alexandra: Does the Pathology of Multiple System Atrophy manifest in the Neuroretina?
    9th ÖGMBT Annual Meeting & 8th Life Science Meeting Innsbruck, Innsbruck, 2017-09-26.

  • Lecturer(s): Lamberti, G. Co-author(s): Laschober, G.; Sopper, S.; Sandbichler, A.M.; Trajanoski, Z.: Metabolic characterization of human T-cells during differentiation.
    9th ÖGMBT Annual Meeting & 8th Life Science Meeting Innsbruck, Innsbruck, 2017-09-26. (Web link)

  • Lecturer(s): Sandbichler, A.M. Co-author(s): Vorhauser, J.; Geng, A.: Peroxisomes and their role in cellular redox balance in zebrafish fibroblasts.
    9th ÖGMBT Annual Meeting & 8th Life Science Meeting Innsbruck, Innsbruck, 2017-09-26. (Web link)

  • Lecturer(s): Kaehler, Kathrin Co-author(s): Seitter, Hartwig; Sandbichler, Adolf; Stefanova, Nadia; Koschak, Alexandra: Does the Pathology of Multiple System Atrophy manifest in the Neuroretina?
    15th Austrian Neuroscience Association (ANA), Klosterneuburg, 2017-09-25. (Web link)

  • Lecturer(s): Kaehler, Kathrin Co-author(s): Seitter, Hartwig; Tschugg, Bettina; Sturm, Edith; Sandbichler, Adolf M.; Stefanova, Nadia; Koschak, Alexandra: The Neuroretina in Multiple System Atrophy.
    5th Annual SFB Meeting 2016, Vill, 2016-11-30. (Web link)

  • Lecturer(s): Kaehler, Kathrin Co-author(s): Seitter, Hartwig; Tschugg, Bettina; Sturm, Edith; Sandbichler, Adolf M.; Stefanova, Nadia; Koschak, Alexandra: The Neuroretina in Multiple System Atrophy.
    22nd Scientific Symposium of the Austrian Pharmacological Society (APHAR), Wien, 2016-09-09. (Web link)

  • Lecturer(s): Kaehler, Kathrin Co-author(s): Seitter, Hartwig; Tschugg, Bettina; Sturm, Edith; Sandbichler, Adolf M.; Stefanova, Nadia; Koschak, Alexandra: The Neuroretina in Multiple System Atrophy: Atrophy Morphological and Functional Aspects in Plp-α-Syn Mice.
    10th FENS Forum of Neuroscience, Kopenhagen, 2016-07-06. (Web link)

  • Lecturer(s): Kaehler, Kathrin Co-author(s): Seitter, Hartwig; Tschugg, Bettina; Sturm, Edith; Sandbichler, Adolf M.; Stefanova, Nadia; Koschak, Alexandra: Retinal immunoreactivity in a mouse model of multiple system atrophy.
    6th Annual CMBI Meeting Innsbruck, Gnadenwald, 2016-03-04. (Web link)

  • Lecturer(s): Kaehler, K. Co-author(s): Seitter, H.; Knoflach, D.; Sturm, E.; Sandbichler, A.; Stefanova, N.; Koschak, A.: Retinal pathomorphology in a mouse model of multiple system atrophy.
    European Retina Meeting 2015, Brighton, 2015-10-01. (Web link)

  • Lecturer(s): Muik, M. Co-author(s): Gomez-Brandon, M.; Sandbichler, A.M.; Insam, H.; Wett, B.; Podmirseg, Sabine Marie: Characterization of anammox granules from a full-scale WWTP by microscopic techniques and a novel particle tracking method.
    8th International Symposium on Anaerobic Microbiology (ISAM8), Innsbruck, 2013-06-12.

  • Lecturer(s): Sandbichler, A.M. Co-author(s): Scheidnagl, S.; Selcuk, D.; Rainer, D.; Pelster, B.; Egg, M.: Mitochondrial plasticity in zebrafish fibroblasts exposed to hypoxia.
    Mitochondrial Trafficking and Function in Health and Disease, Boston, 2012-06-25. (Web link)

  • Lecturer(s): Schredelseker, T. Co-author(s): Schneebauer, G.; Sandbichler, A.M.; Meyer, D.; Schwerte, T.: Analysis of mechanisms to survive oxygen deprivation in vivo and in vitro.
    Swiss and Upper Rhine Valley (SURV) Zebrafish Meeting 2012 (Zebrafish 2012), Basel, 2012-04-13. (Web link)

  • Lecturer(s): Sandbichler, A.; Schwerte, T.; Pelster, B.: Cortisol-dependent remodelling of tight junction claudin 28b and cortical F-actin in cultured gill epithelia of rainbow trout.
    26th ESCPB Congress of the new European Society for Comparative Physiology and Biochemistry, Innsbruck, 2009-09-06.

  • Lecturer(s): Sandbichler, AM.; Schwerte, T.; Pelster, B.: Osmolarity-dependent remodelling of barrier properties in trout gill cell layers.
    4th Annual Meeting of the Center of Molecular Biosciences (CMBI), Igls, 2007-09-28.

  • Lecturer(s): Aigner, M.; Botta, M.; Sandbichler, A.; Salvenmoser, W.; Egger, B.; Rieger, R.; Gschwentner, R.: Development of a basal medium for primary cell culture of marine flatworms.
    10th International Symposium on Flatworm Biology (ISFB 2006), Innsbruck, 2006-07-29.

  • Lecturer(s): Sandbichler, A.; Egg, M.; Pelster, B.: The influence of apical hypoosmotic stress on the expression of cytoskeletal components in trout gill epithelial cells.
    7th International Congress on the Biology of Fish (ICBF VII), St. John’s, 2006-07-18.

  • Lecturer(s): Sandbichler, A.; Egg, M.; Pelster, B.: Transcriptional response to apical osmotic stress in trout gill epithelial cells.
    2nd Annual Meeting of the Center for Molecular Biosciences (CMBI), Vill, 2005-09-30.

  • Lecturer(s): Sandbichler, A.; Egg, M.; Pelster, B.: Transcriptional response to apical osmotic stress in trout gill epithelial cells.
    SEB Annual Main Meeting 2005, Barcelona, 2005-07-11.



Projects, scholarships and other third-party funds

Research Projects

  • Sandbichler, Adolf: Reactive Oxygen Species and their role in the interaction between Circadian Clock and Hypoxic Signaling Pathway.
    Leopold-Franzens-Universität Innsbruck - Vizerektorat für Forschung, Nachwuchsfördermittel aus der Nachwuchsförderung der LFU
    2016-01-01 - 2018-05-31

  • Sandbichler, Adolf: Anpassung von Kiemenepithelzellen an osmotische Veränderungen.
    Tiroler Wissenschaftsförderung (TWF)
    2005-11-03 - 2008-02-29


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