Live Cell Imaging, Signal and Data Analysis

Life cell imaging and measurements in live cells are  important tools in biological research. With the emergence of genetically-encoded fluorescence proteins and sensors it is now possible to selectively tag organelles and proteins within living cells. Our expertise in these research methods is growing and student training is committed to provide a high standard in this field.
Our facility includes a Leica TCS SP5 II confocal microscope, a Zeiss LSM 510 microscope, a video measurement setup (Till Photonics, Polychrome), a live-cell physiometer (Bionas 2500) and a multimode plate reader (Enspire, Perkin Elmer).

 

lci-collage

Reorientation of muscle fibres during asexual bud formation in a lifeact-transgenic Hydra polyp (courtesy Hobmayer group) IdU/CldU pulse-chase-pulse experiment showing stem cells in Macrostomum lignano (courtesy Ladurner group); Motoneurons labeled with GFP in living zebrafish embryos (courtesy Meyer group, Inst. of Molecular Biology), Acetylated tubulin in zebrafish cells (courtesy Aanstad group, Inst. of Molecular Biology), Mitochondria in zebrafish cells labelled with a fluorescent protein (courtesy Pelster group)

Leica SP5 II

Leica TCS SP5 II

Leica TCS SP5 II

The Leica TCS SP5 II is an inverse laser scanning microscope that covers both high resolution 3D imaging and high speed acquisition. Our machine is equipped with 405, 458, 476, 488, 496, 514, 561, 633 nm excitation wavelengths, and 4 channel filter-free AOBS (Acousto-Optical-Beam-Splitter) detection (3 PMT, 1 HyD). The system also has a motorized stage for "mark & find" as well as "tile scan" modes and delivers bright, noise-free images with minimal photo damage at high speed.

We are asking for usage fees on the Leica SP5 II machine. This is necessary to cover upcoming costs for laser replacements, our service plan and consumables.

The fees are 15 €/hour or 40 €/4 hours, incl. tax.

Registered users can book the machine here.

Users should also subscribe to the mailing list lsmusers@lists.uibk.ac.at for updates and announcements by sending an e-mail with the subject "subscribe lsmusers Firstname Lastname" to sympa@lists.uibk.ac.at.

 

signal analysis 1 Signal analysis

Signal analysis techniques and data-mining enable the description and characterization of dynamic biological systems (e.g. cardiac function, muscle movement, cell migration) and its dynamic qualities on the basis of image analyses. One understands the application of (statistical mathematical) methods by data-mining on a data stock with the aim of the pattern recognition. Main fields of application of these techniques are the non-invasive quantification of physiological and developmental processes as well as the quantification of whole animal motions in aquatic models.


For further information please contact: