MACI
Metabolic Analysis Cluster Innsbruck

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In the life sciences, investigations of metabolic pathways, especially of energy metabolism, are gaining an increasingly important role, for example in tumor research, aging research, regeneration research and immunology, as well as, of course, metabolic physiology in the narrower sense. Technological advances in recent years have enabled increasingly precise measurements of metabolic processes, which provide important new insights into fundamental biological regulatory pathways.

The Metabolic Analysis Cluster Innsbruck, MACI for short, bundles the existing expertise and equipment at the University of Innsbruck and represents a competence center for metabolic research, with a focus on the analysis of mitochondrial and other metabolic pathways.

Currently, the center consists of two complementary subcenters, on the one hand a facility at the Rennweg 10 (Institut für biomedizinische Alternsforschung, IBA) for high-resolution respirometry (HRR), for which two oxygraphs (Oxygraph 2K from Oroboros Instruments) are initially available; on the other hand, a facility for physiological flux measurements (metabolic flux analysis, MFA) at the technology site (Institut für Zoologie), for which a flux analyzer (Seahorse XFp from Agilent Technologies) is currently available.

 

oxygraphHigh resolution respirometry (Oxygraph 2K from Oroboros Instruments) at the IBA.

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lcmsMikrophysiometer (Seahorse XFp, Agilent Technologies) und UPLC/MS Anlage (Aquity Arc QDa, Waters) am Institut für Zoologie.


 

Our instrumentation will be upgraded since October 2019 by a modern UPLC/MS (Waters Aquity Arc with Single QDa) at the Institute of Zoology, which will be used mainly for the quantification of various metabolites.

The acquisition of the Seahorse instrument was also supported by the Institute of Molecular Biology (AG Edenhofer) and the UPLC/MS instrument by the Institute of Biochemistry (AG Thedieck) financially supported.

Furthermore, so-called "cell hotels" have been set up at both sites for external users, i.e. suitable incubators that are available for the preparation and, if necessary, post-processing of the respective experiments.


 

For more information please contact:

Pidder Jansen-Dürr (IBA)

Adolf Sandbichler (Institut für Zoologie)

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