Live Cell Imaging, Signal and data analysis

Live cell imaging and measurements on living cells are important tools in biological research. By using genetically encoded fluorescence proteins and sensors it is now possible to selectively label organelles and proteins in living cells. Our know-how in these research methods is reflected in research and teaching at the Institute of Zoology. Currently, we operate a Leica SP5 II LSM, a video measurement stand (Till Photonics, Polychrome V), a cell physiometer (Seahorse XFp) and a multimode platereader (Enspire, Perkin Elmer).



Reorientation of muscle fibres during asexual budding in a lifeact-transgenic Hydra polyp (see AG Hobmayer) IdU/CldU pulse-chase-pulse experiment showing stem cells in Macrostomum lignano (see AG Ladurner); GFP-labeled motoneurons in living zebrafish embryos (see AG Meyer, Inst. for Molecular Biology); acetylated tubulin in zebrafish cells (see AG Aanstad, Inst. for Molecular Biology); GFP-labeled mitochondria in zebrafish cells (see AG Pelster) 


Leica SP5 II

 sp5The Leica TCS SP5 II is an inverted laser scanning microscope capable of  capturing high resolution 3D images as well as fast quantitative processes.  Our instrument is equipped with 405, 458, 476, 488, 496, 514, 561, 633 nm  excitation wavelengths, a filter-free design and 4 detection channels (3 PMT, 1  HyD). The system has a motorized stage for "Mark&Find" or "Tile Scan" modes  and delivers noise-free images with little bleaching.

 To match, the setup has a Stage-Top Chamber from Okolab with atmospheric  control of O2, CO2, humidity and temperature. Alternatively, we also have a  CellASIC ONIX live cell imaging device from Merck for microfluidic perfusion of  up to 4 parallel experimental sets.

A fee is charged for the use of the equipment to cover the smooth operation and future costs for laser replacement, service plan and consumables.

The fee is 15 €/hour or 40 €/4 hours, VAT included.

Registrierte Benutzer können das Gerät hier reservieren.

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Another focus is on signal analysis techniques and data mining.signal-b Based on frequency analyses, they enable the description of oscillating physiological systems (e.g., heart function, muscle movement) and thus its dynamic properties. Data mining is the application of (statistical-mathematical) methods to a data set with the goal of pattern recognition.
Main applications of these techniques are the non-invasive measurement of cardiovascular and respiratory parameters, as well as the quantification of swimming movements in aquatic model organisms such as zebrafish, medaka and xenopus.




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