Zooming into the mouse brain- From cytoarchitecture to synaptic connections: Triple immunofluorescence labelings of mouse brain sagittal sections with neuronal markers Ctip2 (red) and Tbr1 (green) and Hoechst to counterstain nuclei (blue). The inset depicts pre- and postsynaptic contacts between cultured hippocampal neurons transfected with mCherry or eGFP (pseudocolored in yellow and magenta).


 

 

Stefanie M. Geisler, PhD Profil Geisler Stefanie

 

University Assistant (Post-Doc)

Email: Stefanie.Geisler@uibk.ac.at
Phone: +43-(0)512-507 - 58844
ORCID: https://orcid.org/0000-0002-8898-0108

 

 Molecular Endocrinology Group

 

 

 

 

Research interest

Voltage-gated calcium channels (CaVs) provide the primary mechanism for activity-regulated Ca2+ influx into CNS neurons and are thus critical for vitally important functions such as synaptic transmission, cell excitability, and gene expression. Because of their multiple roles and widespread expression any malfunction of these channels can lead to severe neurological disorders including epilepsy, ataxia, and autism spectrum disorders. In my research I am thus aiming to resolve the role of individual CaV subunits and proteins associated with the CaV complex for specific neuronal functions in healthy and diseased nerve cells. During my PhD studies I investigated the role of auxiliary α2δ subunits in synaptogenesis and neurological disease. The central aspect of my thesis revealed that α2δ splice variants regulate the wiring of axons to the GABAergic postsynaptic side and the recruitment of GABAA-receptors and scaffolds (Geisler et al., 2019). As a postdoctoral fellow I am using neuroendocrine chromaffin cells of the adrenal medulla to investigate the role of CaV subunits and CaV-associated proteins in action potential firing and hormone release.

 

Short curriculum

July 2019 - present: Postdoctoral research fellow in the laboratory of Dr. Petronel Tuluc; Department of Pharmacology and Toxicology, University of Innsbruck.

2015-2019: Doctoral studies (PhD) in the Department of Physiology in the laboratory of Dr. Gerald J. Obermair; Medical University of Innsbruck, Austria.

2012-2015: BMA (technical assistant) in the Department of Physiology, Medical University of Innsbruck, Austria.

2009-2012: Master of Science in Animal Biology; University of Salzburg, Austria.

2006-2009: Bachelor studies in natural sciences; University of Innsbruck, Austria.

 

Scientific awards/sholarships/research funding

2021 Tyrolean Science Fund (TWF).

2021 Funding from Lower Austria (NFB). Co-applicant with G.J. Obermair (Karl Landsteiner University of Health Sciences) and R. Shigemoto (IST Austria).

2021 Early stage funding, University of Innsbruck.

2021 Krems Cooperation Research Award - Best thesis award.

2020 Austrian Neuroscience Association (ANA) - Best thesis award.

2017 Austrian Neuroscience Association (ANA) - Travel grant.

2017 Austrian Neuroscience Association (ANA) & MUI Biooptics Facility - Best picture awards.

2015 Austrian Neuroscience Association (ANA) - Travel grant.

2012 Scholarship for funding of my master thesis, University of Salzburg.

2010 Merit scholarship for outstanding academic achievement, University of Salzburg.

 

List of publications

1             Tuluc P, Theiner T, Jacobo-Piqueras N, Geisler SM (2021). Role of High Voltage-Gated Ca2+ Channel Subunits in Pancreatic β-Cell Insulin Release. From Structure to Function. Cells 2021, Vol. 10, Page 2004 10, 2004. doi:10.3390/CELLS10082004.

2             Schöpf CL, Ablinger C*, Geisler SM*, Stanika RI*, Campiglio M, Kaufmann WA, Nimmervoll B, Schlick B, Brockhaus J, Missler M, Shigemoto R, Obermair GJ (2021). Presynaptic α2δ subunits are key organizers of glutamatergic synapses. Proc. Natl. Acad. Sci. 118, e1920827118. doi:10.1073/pnas.1920827118.* equal contribution

3             Geisler SM, Benedetti A, Schöpf CL, Schwarzer C, Stefanova N, Schwartz A, Obermair GJ (2021). Phenotypic Characterization and Brain Structure Analysis of Calcium Channel Subunit α2δ-2 Mutant (Ducky) and α2δ Double Knockout Mice. Front. Synaptic Neurosci. 13. doi:10.3389/fnsyn.2021.634412.

4             El Ghaleb Y, Schneeberger PE, Fernández-Quintero ML, Geisler SM, Pelizzari S, Polstra AM, van Hagen JM, Denecke J, Campiglio M, Liedl KR, Stevens CA, Person RE, Rentas S, Marsh ED, Conlin LK, Tuluc P, Kutsche K, Flucher BE (2021). CACNA1I gain-of-function mutations differentially affect channel gating and cause neurodevelopmental disorders. Brain. doi:10.1093/brain/awab101.

5             Ablinger C, Geisler SM, Stanika RI, Klein CT, Obermair GJ (2020). Neuronal α2δ proteins and brain disorders. Pflugers Arch. Eur. J. Physiol. 472, 845–863. doi:10.1007/s00424-020-02420-2.

6             Geisler S, Schöpf CL, Stanika R, Kalb M, Campiglio M, Repetto D, Traxler L, Missler M, Obermair GJ (2019). Presynaptic α2δ-2 Calcium Channel Subunits Regulate Postsynaptic GABAA Receptor Abundance and Axonal Wiring. J. Neurosci. 39, 2581–2605. doi:10.1523/JNEUROSCI.2234-18.2019. [Journal Cover Image, Featured article (link to: https://www.jneurosci.org/content/39/14/2581.long)]

7             Geisler S*, Schopf CL*, Obermair GJ (2015). Emerging evidence for specific neuronal functions of auxiliary calcium channel alpha(2)delta subunits. Gen Physiol Biophys 34, 105–118. doi:10.4149/gpb_2014037. * equal contribution

8             Daschil N, Geisler S, Obermair GJ, Humpel C (2014). Short- and long-term treatment of mouse cortical primary astrocytes with beta-amyloid differentially regulates the mRNA expression of L-type calcium channels. Pharmacology 93:24–31. doi: 10.1159/000357383.

 

 

 

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