Univ.Prof. Dr. Ronald MICURA
Department of Organic Chemistry
University of Innsbruck
Innrain 80-82
6020 Innsbruck
Austria/Europe
phone: +43 512 507 57710
fax: +43 512 507 57799
Publications
2024
- L. Flemmich, R. Bereiter, R. Micura*; 'Chemical synthesis of modified RNA'; Angew. Chem. Int. Ed. 2024, 63, e202403063.
- K. Bartosik, R. Micura*; 'Access to capped RNA by chemical ligation'; RSC. Chem. Biol. 2024, accepted.
- S. Heel, F. Juen, K. Bartosik, R. Micura, C. Kreutz, K. Breuker*; 'Resolving the intricate binding of neomycin B to multiple binding motifs of a neomycin-sensing riboswitch aptamer by native top-down mass spectrometry and NMR spectroscopy'; Nucleic Acids Res. 2024, 52, 4691-4701.
- M. Kompatscher, K. Bartosik, K. Erharter, R. Plangger, F. S. Juen, C. Kreutz, R. Micura, E. Westhof, M. D. Erlacher*. Contribution of tRNA sequence and modifications to the decoding preferences of E. coli and M. mycoides tRNAGlyUCC for synonymous glycine codons. Nucleic Acids Res. 2024, 52, 1374-1386.
2023
- Y. S. Polikanov*, M. Etheve-Quelquejeu, R. Micura*; 'Synthesis of peptidyl-tRNA mimics for structural biology applications'; Acc. Chem. Res. 2023, 56, 2713-2725.
- C. Eichler #, M. Himmelstoß #, R. Plangger, L. I. Weber, M. Hartl, C. Kreutz*, R. Micura*; 'Advances in RNA labeling with trifluoromethyl groups'; Chem. Eur. J. 2023, 29, e202302220. # these authors contributed equally
- J. Thaler #, E. Syroegin #, K. Breuker, Y. Polikanov*, R. Micura*; 'Practical synthesis of N-formylmethionylated peptidyl-tRNA mimics'; ACS Chem. Biol. 2023, 18, 2233–2239. # these authors contributed equally
- S. Heel, K. Bartosik, F. Juen, C. Kreutz, R. Micura, K. Breuker*; 'Native top-down mass spectrometry uncovers two distinct binding motifs of a functional neomycin-sensing riboswitch aptamer'; J. Am. Chem. Soc. 2023, 145, 15284–15294.
- X. Xu #, M. Egger #, C. Li #, H. Chen, R. Micura*, A. Ren*; 'Structure-based investigations of the NAD+-II riboswitch'; Nucleic Acids Res. 2023, 51, 54–67. # these authors contributed equally
2022
- M. Egger, R. Bereiter, S. Mair, R. Micura*; 'Scaling catalytic contributions of small self-cleaving ribozymes'; Angew. Chem. Int. Ed. 2022, 61, e202207590.
- E. A. Syroegin #, L. Flemmich #, D. Klepacki, N. Vazquez-Laslop, R. Micura*, Y. Polikanov*; 'Structural basis for the context-specific action of the classic peptidyl transferase inhibitor chloramphenicol'; Nat. Struct. Mol. Biol. 2022, 29, 152–161. # these authors contributed equally; see News&Views by C. Crowe-McAuliffe & D. N. Wilson, Putting the antibiotics chloramphenicol and linezolid into context, Nat. Struct. Mol. Biol. 2022, 29, 79–91.
- S. Moreno, M. Brunner, I. Delazer, D. Rieder, A. Lusser,* R. Micura*; 'Synthesis of 4-thiouridines with prodrug functionalization for RNA metabolic labeling'; RSC Chem. Biol. 2022, 3, 447–455.
- S. Moreno, J. M. Ramos Pittol, M. Hartl, R. Micura*; 'Robust synthesis of 2'-azido modified RNA from 2'-amino precursors by diazotransfer reaction'; Org. Biomol. Chem. 2022, 20, 7845–7850.
- S. Moreno, M. Fickl, I. Bauer, M. B., A. Rázková, D. Rieder, I. Delazer, R. Micura,* A. Lusser*; 6-Thioguanosine monophosphate prodrugs display enhanced performance against thiopurine-resistant leukemia and breast cancer cells, J. Med. Chem. 2022, 65, 15165–15173.
- S. Mair, K. Erharter, E. Renard, K. Brillet, M. Brunner, A. Lusser, C. Kreutz*, E. Ennifar,* R. Micura*; 'Towards a comprehensive understanding of RNA deamination – Synthesis and properties of xanthosine-modified RNA'; Nucleic Acids Res. 2022, 50, 6038–6051.
- R. Bereiter, E. Renard, K. Breuker, C. Kreutz, E. Ennifar, R. Micura*; '1-Deazaguanosine modified RNA – The missing piece for functional RNA atomic mutagenesis'; J. Am. Chem. Soc. 2022, 144, 10344–10352.
- R. Bereiter, M. Oberlechner, R. Micura*; 'A new route for the synthesis of 1-deazaguanine and 1-deazahypoxanthine'; Beilstein. J. Org. Chem. 2022, 18, 1617–1624.
- S. Moreno, L. Flemmich, R. Micura*; 'Synthesis of N4-acetylated 3-methylcytidine phosphoramidites for RNA solid-phase synthesis'; Monatshefte für Chemie - Chemical Monthly, 2022, 153, 285–291.
- L. Bessler, N. Kaur, L.-M- Vogt, L. Flemmich, C. Siebenaller, M.-L. Winz, F. Tuorto, R. Micura, A. Ehrenhofer-Murray,* M. Helm*; 'Functional integration of a semi-synthetic azido-queuosine derivative into translation and a tRNA modification circuit', Nucleic Acids Res. 2022, 50, 10785–10800.
- F. Erhard*, E. Saliba, A. Lusser, C. Toussaint, T. Hennig, B. Prusty, D. Kirschenbaum, K. Abadie, E. Miska, C. Friedel, I. Amit, R. Micura, L. Dölken*; 'Time-resolved single-cell RNA-seq using metabolic RNA labeling', Nat. Rev. Methods Primers 2022, 2, 77.
- M. Mitter*, Z. Takacs, T. Köcher, R. Micura, C. Langer, D. Gerlich*; 'Sister-chromatid-sensitive Hi-C to map the conformation of replicated genomes'; Nat. Protoc. 2022, 17, 1486–1517.
2021
- L. Flemmich, S. Heel, S. Moreno, K. Breuker, R. Micura*; 'A natural riboswitch scaffold with self-methylation activity'; Nat. Commun. 2021, 12, 3877.
- L. Flemmich, S. Moreno, R. Micura*; 'Synthesis of O6-alkylated preQ1 derivatives'; Beilstein J. Org. Chem. 2021, 17, 2295–2301.
- R. Bereiter #, M. Himmelstoß #, E. Renard, E. Mairhofer, M. Egger, K. Breuker, C. Kreutz, E. Ennifar, R. Micura*; 'Impact of 3-deazapurine nucleobases on RNA properties'; Nucleic Acids Res. 2021, 49, 4281–4293. # these authors contributed equally
- X. Xu #, M. Egger #, H. Chen #, K. Bartosik, R. Micura*, A. Ren*; 'Insights into xanthine riboswitch structure and metal ion-mediated ligand recognition'; Nucleic Acids Res. 2021, 49, 7139 - 7153. # these authors contributed equally
- O. A. Krasheninina, J. Thaler, M. E. Erlacher, R. Micura*; 'Amine-to-azide conversion on native RNA via metal-free diazo transfer opens new avenues for RNA manipulations'; Angew. Chem. Int. Ed. 2021, 60, 6970-6974.
- C. Falschlunger, J. Leiter, R. Micura*; 'Elucidation of Ribozyme Mechanisms at the Example of the Pistol Ribozyme', in Ribozymes - Principles, Methods, Applications, Eds.: S. Müller, B. Masquida, W. Winkler, Wiley-VCH, Weinheim, 2021.
2020
- R. Micura,* C. Höbartner*; 'Fundamental studies of functional nucleic acids: aptamers, riboswitches, ribozymes and DNAzymes'; Chem. Soc. Rev. 2020, 7331-7535.
- M. Himmelstoß, K. Erharter, E. Renard, E. Ennifar,* C. Kreutz,* R. Micura*; '2′-O-Trifluoromethylated RNA – A powerful modification for RNA chemistry and NMR spectroscopy'; Chem. Sci. 2020, 11, 11322-11330.
- C. Gasser #, I. Delazer #, E. Neuner +, K. Pascher +, K. Brillet, S. Klotz, L. Trixl, M. Himmelstoß, E. Ennifar, D. Rieder,* A. Lusser,* R. Micura*; 'Thioguanosine conversion enables mRNA life-time evaluation by RNA sequencing via double metabolic labeling (TUC-seq DUAL)'; Angew. Chem. Int. Ed. 2020, 59, 6881-6886. #,+ these authors contributed equally
- M. Teplova #, C. Falschlunger #, O. Krasheninina #, M. Egger, A. Ren, D. J. Patel,* R. Micura*; 'On crucial roles of two hydrated Mg2+ ions in reaction catalysis of the pistol ribozyme'; Angew. Chem. Int. Ed. 2020, 59, 2837-2843. # these authors contributed equally
- H. Chen #, M. Egger #, X. Xu #, L. Flemmich, O. Krasheninina, A. Sun, R. Micura,* A. Ren*; 'Structural distinctions between NAD+ riboswitch domains 1 and 2 determine differential folding and ligand binding'; Nucleic Acids Res. 2020, 48, 12394–12406. # these authors contributed equally
- ,* , , , , , , , , , , , Nature 2020, 586, 139-144.
- J. Leiter, D. Reichert, A. Rentmeister, R. Micura*; 'Practical Synthesis of Cap-4 RNA'; ChemBioChem 2020, 21, 265-271.
- A. Lusser*, C. Gasser, L. Trixl, P. Piatti, D. Rieder, J. Bashin, I. Delazer, C. Riml, T. Amort, R. Micura*; 'Thiouridine-to-Cytidine conversion sequencing (TUC-seq) to measure mRNA transcription and degradation rates'; Meth. Mol. Biol. 2020, 2062, 191-211.
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S. Werner, L. Schmidt, V. Marchand, T. Kemmer, C. Falschlunger, M. V. Sednev, G. Bec, E. Ennifar, C. Höbartner, R. Micura, Y. Motorin, A. Hildebrandt, M. Helm*; Machine learning of reverse transcription signatures of variegated polymerases allows mapping and discrimination of methylated purines in limited transcriptomes'; Nucleic Acids Res. 2020, 48, 3734-3746.
2019
- A. Sun #, C. Gasser #, F. Li #, H. Chen, S. Mair, O. Krasheninina, R. Micura,* A. Ren*; 'SAM-VI riboswitch structure and signature for ligand discrimination'; Nat. Commun. 2019, 10, 5728. # these authors contributed equally
- E. Mairhofer, L. Flemmich, C. Kreutz, R. Micura*; 'Access to 3-Deazaguanosine Building Blocks for RNA Solid-Phase Synthesis Involving Hartwig-Buchwald C-N Cross-Coupling'; Org. Lett., 2019, 21, 3900-3903.
- L. Zheng #, C. Falschlunger #, K. Huang, E. Mairhofer, S. Yuan, J. Wang, Dinshaw J. Patel*, R. Micura*, A. Ren*; 'Hatchet ribozyme structure and implications for cleavage mechanism'; Proc. Nat. Acad. Sci. 2019, 116, 10783-10791. # these authors contributed equally
- S.V. Melnikov, N.F. Khabibullina, E. Mairhofer, O. Vargas-Rodriguez, N.M. Reynolds, R. Micura,* D. Söll*, Y.S. Polikanov*; 'Mechanistic insights into the slow peptide bond formation with D-amino acids in the ribosomal active site'; Nucl. Acids Res. 2019, 47, 2089-2100.
- E. Fuchs, C. Falschlunger, R. Micura, K. Breuker*; 'The effect of adenine protonation on RNA phosphodiester backbone bond cleavage elucidated by deaza-nucleobase modifications and mass spectrometry'; Nucl. Acids Res. 2019, 47, 7223-7234.
- C. Falschlunger, R. Micura*; 'Efficient access to N‐trifluoroacetylated 2′‐amino‐2′‐deoxyadenosine phosphoramidite for RNA solid‐phase synthesis'; Monatshefte für Chemie - Chemical Monthly, 2019, 150, 795-800.
- E. Neuner, R. Micura*; 'Practical synthesis N-(di-n-butylamino)methylene protected 2-aminopurine riboside phosphoramidite for RNA solid-phase synthesis'; Monatshefte für Chemie - Chemical Monthly, 2019, 1941–1946.
- C. Dégut, V. Schwarz, L. Ponchon, P. Barraud, R. Micura, C. Tisné*; 'Design of cross-linked RNA/protein complexes for structural studies'; Biochimie, 2019, 164, 95-98.
- S. Mukherjee, L. Błaszczyk, W. Rypniewski, C. Falschlunger, R. Micura, A. Murata, C. Dohno, K. Nakatani*, A. Kiliszek*; 'Structural insights into synthetic ligands targeting A-A pairs in disease-related CAG RNA repeats'; Nucl. Acids Res. 2019, 47, 10906-10913.
2018
- E. Neuner #, M. Frener #, A. Lusser*, R. Micura*; 'Superior cellular activities of azido- over amino-functionalized ligands for engineered preQ1 riboswitches in E.coli'; RNA Biol. 2018, 15, 1376-1383. # these authors contributed equally
- C. Gasser, J. Gebetsberger*, M. Gebetsberger, R. Micura*; 'SHAPE probing pictures Mg2+ dependent folding of small self-cleaving ribozymes'; Nucl. Acids Res. 2018, 46, 6983-6995.
- T.P. Hoernes, K. Faserl, M.A. Juen, J. Kremser, C. Gasser, E. Fuchs, Xi. Shi, A. Siewert, H. Lindner, C. Kreutz, R. Micura, S. Joseph, C. Höbartner, E. Westhof, A. Hüttenhofer, M.D. Erlacher*; 'Translation of non-standard nucleotides reveals minimal requirements for codon-anticodon interactions', Nat. Commun. 2018, 9, 4865.
2017
- S. Neuner #, C. Falschlunger #, E. Fuchs, M. Himmelstoss, A. Ren, D. J. Patel, R. Micura*; 'Atom-specific mutagenesis reveals structural and catalytic roles for active site–adenosine and Mg2+ in pistol ribozyme'; Angew. Chem. Int. Ed. 2017, 56, 16170-16174. # these authors contributed equally
- C. Riml #, T. Amort #, D. Rieder #, C. Gasser, A. Lusser*, R. Micura*; 'Osmium-mediated 4sU-to-C transformation as key to study RNA dynamics by sequencing (TUC-seq)'; Angew. Chem. Int. Ed. 2017, 56, 13479-13483. # these authors contributed equally; see comment: 'RNA dynamics revealed by metabolic RNA labeling and biochemical nucleoside conversions. M. A. P. Baptista, L. Dölken. Nat. Methods 2018,15, 171-172.
- N. Vusurovic #, R. B. Altman #, D. S. Terry, R. Micura*, S. C. Blanchard*; 'Pseudoknot formation seeds the twister ribozyme cleavage reaction coordinate'; J. Am. Chem. Soc. 2017, 139, 8186-8193. # these authors contributed equally
- L. Zheng #, E. Mairhofer #, M. Teplova, J. Ma, D. J. Patel, R. Micura*, A. Ren*; 'Structure-based insights into self-cleavage by a four-way junctional twister-sister ribozyme'; Nat. Commun. 2017, 8, 1180. # these authors contributed equally
- A. Ren, R. Micura, D. J. Patel*, 'Structure-based mechanistic insights into catalysis of small self-cleaving ribozymes'; Curr. Opin. Chem. Biol. 2017, 41, 71-83.
- C. Riml, A. Lusser, E. Ennifar, R. Micura*; 'Synthesis, thermodynamic properties, and crystal structure of RNA oligonucleotides containing 5-hydroxymethylcytosine'; J. Org. Chem. 2017, 82, 7939-7945.
- H. Glasner, C. Riml, R. Micura, K. Breuker*; 'Label-free, direct localization and quantitation of the RNA nucleobase methylations m6A, m5C, m3U, and m5U by top-down mass spectrometry'; Nucl. Acids Res. 2017, 45, 8014-8025.
- J. Gebetsberger, R. Micura*; 'Unwinding the twister ribozyme: From structure to mechanism'; WIREs RNA 2017, 8:e1402. ( Published Online: Nov 14 2016 DOI: 10.1002/wrna.1402 )
- L. Jud, R. Micura*; 'An unconventional acid-labile nucleobase protection concept for guanosine phosphoramidites in RNA solid-phase synthesis'; Chem. Eur. J. 2017, 23, 3406–3413.
- T. Amort, D. Rieder, A. Wille, D. Khokhlova-Cubberley, C. Riml, L. Trixl, X.-Y. Jia, R. Micura, A. Lusser*; 'Distinct 5-methylcytosine profiles in poly(A)RNA from mouse embryonic stem cells and the brain'; Genome Biology, 2017, 18, 1.
- E. M. Danhart, M. Bakhtina, W.A. Cantara, A. B. Kuzmishin, X. Ma, B. L. Sanford, M. Kosutic, Y. Goto, H. Suga, K. Nakanishi, R. Micura, M. P. Foster*, K. Musier-Forsyth*; 'Conformational selection by a trans-acting editing domain'; Proc. Nat. Acad. Sci. 2017, 114, E6774-6783.
- C. Riml, R. Micura; 'Automated chemical solid-phase synthesis and deprotection of 5-hydroxymethylcytosine-containing RNA'; in RNA Methylation (Eds. A. Lusser), Meth. Mol. Biol. 2017, 1562, 295-302.
- S. Neuner, C. Kreutz*, R. Micura*; 'The synthesis of 15N(7)-Hoogsteen face-labeled adenosine phosphoramidite for solid-phase RNA synthesis'; Monatshefte für Chemie - Chemical Monthly, 2017, 148, 149-155. Special Issue "Organic chemistry – Austrian Chemical Society (GÖCH) work group"
2016
- M. Frener, R. Micura*; 'Conformational rearrangements of individual nucleotides during RNA-ligand binding are rate-differentiated'; J. Am. Chem. Soc. 2016, 138, 3627-3630.
- E. Mairhofer #, E. Fuchs #, R. Micura*; 'Facile synthesis of 3-deazaadenosine phosphoramidite for RNA solid-phase synthesis'; Beilstein J. Org. Chem. 2016, 12, 2556-2562. # these authors contributed equally
- A. Ren #, N. Vusurovic #, J. Gebetsberger, P. Gao, M. Juen, C. Kreutz, R. Micura,* D.J. Patel*; 'Pistol ribozyme adopts a pseudoknot fold facilitating site-specific in-line cleavage'; Nat. Chem. Biol. 2016, 12, 702-708. # these authors contributed equally
- S. Melnikov, J. Mailliot, L. Rigger, S. Neuner, B.-S. Shin, G. Yusupova, T. E. Dever,* R. Micura*, M. Yusupov*; 'Molecular insights into protein synthesis with proline residues'; EMBO Reports 2016, 17, 1776-1784.
- S. Sothiselvam, S. Neuner, L. Rigger, D. Klepacki, R. Micura, N. Vázquez-Laslop,* A. S. Mankin*; 'The ribosome corrupted by macrolide antibiotics discriminates against specific substrates based on their charge and size'; Cell Reports 2016, 16, 1789-1799.
- C. Riml, R. Micura*; 'Synthesis of 5-hydroxymethylcytidine and 5-hydroxymethyluridine-modified RNA'; Synthesis 2016, 48, 1108-1116.
- S. Melnikov, J. Mailliot, B.-S. Shin, L. Rigger, G. Yusupova, R. Micura*, T. E. Dever,* M. Yusupov*; 'Crystal structure of hypusine-containing translation fachtor eIF5A bound to a rotated eukaryotic ribosome'; J. Mol. Biol. 2016, 428, 3570-3576.
- S. Flür, R. Micura*; 'Chemical synthesis of RNA with site-specific methylphosphonate modifications'; Methods 2016, 107, 79-88.
2015
- M. Kosutic #, S. Neuner #, A. Ren, S. Flür, C. Wunderlich, E. Mairhofer, N. Vusurovic, J. Seikowski, K. Breuker, C. Höbartner, D.J. Patel,* C. Kreutz,* R. Micura*; 'A Mini-Twister Variant and Impact of Residues/Cations on the Phosphodiester Cleavage of this Ribozyme Class'; Angew. Chem. Int. Ed. 2015, 54, 15128-15233. # these authors contributed equally
- S. Neuner #, T. Santner #, C. Kreutz, R. Micura*; 'The "speedy" synthesis of atom-specific 15N imino/amido-labeled RNA'; Chem. Eur. J. 2015, 21, 11634-11643. # these authors contributed equally.
- L. Jud #, M. Kosutic #, V. Schwarz, M. Hartl, C. Kreutz, K. Bister, R. Micura*; 'Expanding the scope of 2'-SCF3 modified RNA'; Chem. Eur. J. 2015, 21, 10400-10407. # these authors contributed equally.
- M. Koch #, S. Flür #, C. Kreutz, R. Micura*, N. Polacek*; 'The role of a ribosomal RNA phosphate oxygen during the EF-G-triggered GTP hydrolysis'; Proc. Nat. Acad. Sci. 2015, 112, E6256–E6259. # these authors contributed equally.
- C. Riml, H. Glasner, M. T. Rodgers, R. Micura, K. Breuker*; 'On the mechanism of RNA phosphodiester backbone cleavage in the absence of solvent'; Nucl. Acids Res. 2015, 43, 5171-5181.
- T. Moschen, C. H. Wunderlich, R. Spitzer, J. Levic, R. Micura, M. Tollinger*, C. Kreutz*; 'Ligand-Detected Relaxation Dispersion NMR Spectroscopy: Dynamics of preQ1–RNA Binding'; Angew. Chem. Int. Ed. 2015, 570-573.
- A.-S. Geiermann, R. Micura; 'Native chemical Ligation of hydrolysis-resistant 3'-NH-cystein-modified RNA'; Curr. Protoc. Nucl. Acid Chem. 2015, Wiley, 62:4.64.1-4.64.36.
2014
- A. Ren #, M. Kosutic #, K. R. Rajashankar, M. Frener, T. Santner, E. Westhof, R. Micura*, D. J. Patel*; 'In-line alignment and Mg2+ coordination at the cleavage site of the env22 twister ribozyme'; Nat. Commun. 2014, 5: 5534. # these authors contributed equally; Pascal Legault, Faculty of 1000 Structural Biology.
- S. Neuner, R. Micura*; 'Synthesis of aminoacetylated N6,N6-dimethyladenosine solid support for efficient access to hydrolysis-resistant 3'-charged tRNA mimics'; Bioorg. Med. Chem. 2014, 22, 6989-6995.
- F. Spenkuch, G. Hinze, S. Kellner, C. Kreutz, R. Micura, T. Basché, M. Helm*; 'Dye label interference with RNA modification reveals 5-fluorouridine as non-covalent inhibitor'; Nucl. Acids Res. 2014, 42, 12735-12745.
- J. Levic, R. Micura*; 'Syntheses of 15N-labeled pre-queuosine base derivatives'; Beilstein J. Org. Chem. 2014, 10, 1914-1918.
- M. Kosutic #, L. Jud #, C. Da Veiga, M. Frener, K. Fauster, C. Kreutz, E. Ennifar*, R. Micura*; 'Surprising base pairing and structural properties of 2'-Trifluoromethylthio-Modified RNA'; J. Am. Chem. Soc. 2014, 136, 6656-6663. # these authors contributed equally
- T. Santner, M. Hartl, K. Bister, R. Micura*; 'Efficient access to 3'-terminal azide-modified RNA for inverse Click-labeling patterns'; Bioconj. Chem. 2014, 25, 188-195.
- M. F. Soulière*, R. Micura*; 'Use of SHAPE to select 2AP substitution sites for RNA-ligand interactions and dynamics studies'; in Therapeutic applications of ribozymes and riboswitches (Eds. D. Lafontaine, A. Dubé), Meth. Mol. Biol. 2014, 1103, 227-239.
2013
- M. F. Soulière, R. B. Altman, V. Schwarz, A. Haller, S. C. Blanchard*, R. Micura*; 'Tuning a riboswitch response through structural extension of a pseudoknot'; Proc. Nat. Acad. Sci. 2013, 110, E3256-E3264.
- R. Micura*; 'RNA biophysics: A three-state balancing act'; Nature 2013, 499, 289-290 (News&Views).
- L. Rigger, R. L. Schmidt, K. M. Holman, M. Simonovic*, R. Micura*; 'The synthesis of methylated, phosphorylated, and phosphonated 3'-aminoacyl-tRNASec mimics'; Chem. Eur. J. 2013, 19, 15872-15878. (Front cover)
- M. Duval, A Korepanov, O. Fuchsbauer, P. Fechter, A. Haller, A. Fabbretti, L. Choulier, R. Micura, B. Klaholz, P. Romby*, M. Springer, S. Marzi*; 'Escherichia coli ribosomal protein S1 unfolds structured mRNA onto the ribosome for active translation initiation'; PLoS Biology 2013, 11(12), e1001731.
- G. Bec, B. Meyer, M.-A. Gerard, J. Steger, K. Fauster, P. Wolff, D. Bernouf, R. Micura, P. Dumas, E. Ennifar*; 'Thermodynamics of HIV-1 reverse transcriptase in action elucidates the mechanism of action of non-nucleoside inhibitors'; J. Am. Chem. Soc. 2013, 135, 9743–9752.
- R. Micura*, C. Kreutz*, K. Breuker*; 'A personal perspective on chemistry-driven RNA research'; Biopolymers 2013, 99, 1114-1123.
- T. Amort, M. F. Soulière, A. Wille, X.-Y. Jia, H. Fiegl, H. Wörle, R. Micura, A. Lusser*; 'Long non-coding RNAs as targets for cytosine methylation'; RNA Biology 2013, 10, 1002-1008.
- A. Haller, R. B. Altman, M. Soulière, S. C. Blanchard*, R. Micura*; 'Folding and ligand recognition of the TPP riboswitch aptamer at single-molecule resolution'; Proc. Nat. Acad. Sci. 2013, 110, 4188-4193.
- M. F. Soulière, A. Haller, T. Santner, R. Micura*; 'New Insights into Gene Regulation - High Resolution Structures of Cobalamin Riboswitches'; Angew. Chem. Int. Ed. 2013, 52, 1874-1877; Angew. Chem. 2013, 125, 1924-1927.
2012
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K. Fauster, C. Kreutz, R. Micura*; '2'-SCF3 uridine - A novel powerful label for probing RNA structure and function by 19F-NMR spectroscopy'; Angew. Chem. Int. Ed. 2012, 51, 13080-13084; Angew. Chem. 2012, 124,13257-13261
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V. Siegmund, T. Santner, R. Micura, A. Marx*; 'Screening mutant libraries of T7 RNA polymerase for candidates with increased acceptance of 2'-modified nucleotides'; Chem. Comm. 2012, 48, 9870-9872
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T. Santner #, U. Rieder #, C. Kreutz*, R. Micura*; 'Pseudoknot preorganization of the preQ1 class I riboswitch'; J. Am. Chem. Soc. 2012, 134, 11928-11931. # these authors contributed equally
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A.-S. Geiermann, R. Micura*; 'Selective desulfurization significantly expands sequence variety of 3’-peptidyl-tRNA mimics obtained by native chemical ligation'; Chembiochem 2012, 12,1742-1745
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T. Santner, V. Siegmund, A. Marx, R. Micura*; The synthesis of 2'-methylseleno adenosine and guanosine 5'-triphosphates; Bioorg. Med. Chem. 2012, 20, 2416-2418. (Front cover)
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K. Fauster, M. Hartl, T. Santner, M. Aigner, C. Kreutz, K. Bister*, E. Ennifar*, R. Micura*; 2-Azido RNA, a Versatile Tool for Chemical Biology: Synthesis, X-ray Structure, siRNA Applications, Click Labeling; ACS Chem. Biol. 2012, 7, 581-589. (Front cover)
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R. Micura; book review on 'Metal Ions in Life Sciences, Vol. 9., Structural and Catalytic Roles of Metal Ions in RNA', A. Sigel, H. Sigel, R.K.O. Sigel (Eds.) RSC Publishing (2011); Coord. Chem. Rev. 2012, 256, 370
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D. Graber, K. Trappl, J. Steger, A.-S. Geiermann, L. Rigger, H. Moroder, N. Polacek, R. Micura*; 'Deoxyribozyme-based, semisynthetic access to stable peptidyl-tRNAs exemplified by tRNA(Val) carrying a macrolide antibiotic resistance peptide'; in Ribozymes (Ed. J. S. Hartig), Meth. Mol. Biol. 2012, 848, 201-213.
2011
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A.-S. Geiermann, N. Polacek, R. Micura*; 'Native chemical ligation of hydrolysis-resistant 3’-peptidyl-tRNA mimics'; J. Am. Chem. Soc. 2011, 133, 19068-19071.
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M. Soulière, A. Haller, R. Rieder, R. Micura*; 'A powerful approach for the selection of 2-aminopurine substitution sites to investigate RNA folding'; J. Am. Chem. Soc. 2011, 133, 16161-16167
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V. Siegmund, T. Santner, R. Micura*, A. Marx*; 'Enzymatic synthesis of 2'-methylseleno-modified RNA'; Chem. Sci. 2011, 2, 2224-2231
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J. Steger, R. Micura*; 'Functionalized polystyrene supports for solid-phase synthesis of glycyl-, alanyl-, and isoleucyl-RNA conjugates as hydrolysis-resistant mimics of peptidyl-tRNAs'; Bioorg. Med. Chem. 2011, 19, 5167-5174
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A. Haller #, M. F. Soulière #, R. Micura*; 'The dynamic nature of RNA as key to understanding riboswitch mechanisms'; Acc. Chem. Res. 2011, 44, 1339-1348. # these authors contributed equally
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A. Haller, U. Rieder, M. Aigner, S. C. Blanchard*, R. Micura*; 'Conformational capture of the SAM-II riboswitch'; Nat. Chem. Biol. 2011, 7, 393-400.
News&Views by Ming C. Hammond; 'RNA folding: A tale of two riboswitches'; Nat. Chem. Biol. 2011, 7, 342-343 -
H. Ramu, N. Vázquez-Laslop, D. Klepacki, Q. Dai, J. Piccirilli, R. Micura, A. S. Mankin*; 'Nascent Peptide in the Ribosome Exit Tunnel Affects Functional Properties of the A-Site of the Peptidyl Transferase Center'; Mol. Cell. 2011, 41, 321-330.
Preview by D. N. Wilson*; 'Peptides in the Ribosomal Tunnel Talk Back'; Mol. Cell 2011, 41, 247-248 -
M. Aigner, M. Hartl, K. Fauster, J. Steger, K. Bister, R. Micura*; 'Chemical Synthesis of Site-Specifically 2'-Azido-Modified RNA and Potential Applications for Bioconjugation and RNA Interference'; Chembiochem 2011, 11, 47-51
2010
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J. Steger, D. Graber, H. Moroder, A.-S. Geiermann, M. Aigner, R. Micura*; 'Efficient Access to Nonhydrolyzable Initiator tRNA Based on the Synthesis of 3′-Azido-3′-Deoxyadenosine RNA'; Angew. Chem. Int. Ed. Engl. 2010, 49, 7470-7472; Angew. Chem. 2010, 122, 7632-7634
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Y. Wang, J. Ludwig, C. Schuberth, M. Goldeck, M. Schlee, H. Li, S. Juranek, G. Sheng, R. Micura, T. Tuschl, G. Hartmann, D. J. Patel; 'Structural and functional insights into 5'-ppp RNA pattern recognition by the innate immune receptor RIG-I'; Nat. Struct. Mol. Biol. 2010, 17, 781-787.
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D. Graber, H. Moroder, J. Steger, K. Trappl, N. Polacek, R. Micura*; 'Efficient semisynthesis of hydrolysis-resistant 3'-peptidyl-tRNA conjugates containing genuine tRNA modifications'; Nucl. Acids Res. 2010, 38, 6796-6802.
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U. Rieder, C. Kreutz, R. Micura*; 'Folding of a transcriptionally acting preQ1 riboswitch'; Proc. Natl. Acad. Sci. U.S.A. 2010, 107, 10804-10809.
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N. Clementi, A. Chirkova, B. Puffer, R. Micura, N. Polacek*; 'Atomic mutagenesis reveals A2660 of 23S ribosomal RNA as key to EF-G GTPase activation'; Nat. Chem. Biol. 2010, 6, 344-351.
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A. Chirkova, M. Erlacher, R. Micura, N. Polacek*; 'Chemically engineered ribosomes: A new frontier in synthetic biology'; Curr. Org. Chem. 2010, 14, 148-161.
2009
- A.-S. Geiermann, R. Micura*; 'Chasing after antibiotic leads'; Chem. Biol. 2009, 16, 1024-1025 (Preview).
- B. Puffer, C. Kreutz, U. Rieder, M.-O. Ebert, R. Konrat, R Micura*; '5-Fluoro pyrimidines: Labels to probe DNA and RNA secondary structures by 1D 19F NMR spectroscopy'; Nucl. Acids Res. 2009, 37, 7728-7740.
- K. De Mulder, D. Pfister, G. Kuales, B. Egger, W. Salvenmoser, M. Willems, J. Steger, K. Fauster, R. Micura, G. Borgonie, P. Ladurner*; 'Stem cells are differentially regulated during development, regeneration and homeostasis in flatworms'; Dev. Biol. 2009, 334, 198-212.
- U. Rieder, K. Lang, C. Kreutz, N. Polacek, R. Micura*; 'Evidence for Pseudoknot Formation of Class I preQ1 Riboswitch Aptamers'; Chembiochem 2009, 10, 1141-1144.
- H. Moroder #, J. Steger #, D. Graber, K. Fauster, K. Trappl, V. Marquez, N. Polacek, D. N. Wilson, R. Micura*; 'Nonhydrolyzable RNA–Peptide Conjugates: A Powerful Advance in the Synthesis of Mimics for 3'-Peptidyl tRNA Termini'; Angew. Chem. Int. Ed. Engl. 2009, 48, 4056-4060; Angew. Chem. 2009, 121, 4116-4120. # these authors contributed equally
- V. Olieric #, U. Rieder #, K. Lang, A. Serganov, C. Schulze-Briese, R. Micura, P. Dumas, E. Ennifar*; 'A fast strategy for selenium derivatization directed to phasing of X-ray RNA structures'; RNA 2009, 15, 707-715.# these authors contributed equally
- R. Rieder, C. Höbartner, R. Micura*; 'Enzymatic ligation strategies for the preparation of purine riboswitches with site-specific chemical modifications', in Riboswitches (Ed. A. Serganov), Meth. Mol. Biol. 2009, 540, 15-24.
2008
- D. Graber, H. Moroder, R. Micura*; '19F NMR spectroscopy for the analysis of RNA secondary structure populations'; J. Am. Chem. Soc. 2008, 130, 17230–17231.
- P. S. Pallan, C. Kreutz, S. Bosio, R. Micura*, Martin Egli*; 'Effects of N2,N2-dimethylguanosine on RNA structure and stability: crystal structure of an RNA duplex with tandem m22G:A pairs', RNA 2008, 14, 2125-2135.
- K. Lang, R. Micura*; 'Preparation of site-specifically modified riboswitch domains as an example for enzymatic ligation of chemically synthesized RNA fragments', Nature Protocols 2008, 3, 1457-1466.
- K. Lang, M. Erlacher, D. N. Wilson, R. Micura*, N. Polacek*; 'The role of 23S rRNA residue A2451 in ribosomal peptide bond synthesis revealed by atomic mutagenesis'; Chem. Biol. 2008, 15, 485-492.
Preview by M. Pech, K. H. Nierhaus*; 'Ribosomal peptide-bond formation'; Chem. Biol. 2008, 15, 417-419. - S. Freisz, K. Lang, R. Micura, P. Dumas, E. Ennifar*; 'Binding of aminoglycoside antibiotics to the duplex form of the HIV-1 genomic RNA dimerization initiation site'; Angew. Chem. 2008, 120, 4178-4181; Angew. Chem. Int. Ed. Engl. 2008, 47, 4110-4113.
- B. Puffer, H. Moroder, M. Aigner, R. Micura*; '2'-Methylseleno modified oligoribonucleotides for X-ray crystallography synthesized by the ACE RNA solid-phase approach'; Nucl. Acids Res. 2008, 36, 970-983.
- C. Kreutz, R. Micura*; 'Investigations on fluorine-labeled ribonucleic acids by 19F NMR spectroscopy'; Modified Nucleotides in Biochemistry, Biotechnology and Medicine; Ed.: P. Herdewijn, Wiley-VCH, 2008, 3-22.
- R. Micura; contributions to 'Nucleic Acids from A to Z – A Concise Encyclopedia'; Ed.: Sabine Müller, Wiley-VCH, 2008.
2007
- F. Li, P. Pallan, M. Maier, K. Rajeev, S. Mathieu, C. Kreutz, Y. Fan, J. Sanghvi, R. Micura, E. Rozners, M. Manoharan, M. Egli*; 'Crystal structure, stability and in vitro RNAi activity of oligoribonucleotides containing the ribo-difluorotoluyl nucleotide: insights into substrate requirements by the human RISC Ago2 enzyme'; Nucl. Acids Res. 2007, 35, 6424-6438.
- K. Lang, R. Rieder, R. Micura*; 'Ligand-induced folding of the thiM TPP riboswitch investigated by a structure-based fluorescence spectroscopic approach'; Nucl. Acids Res. 2007, 35, 5370-5378.
- M. Amort, B. Wotzel, K. Bakowska-Zywicka, M. D. Erlacher, R. Micura, and N. Polacek*; 'An intact ribose moiety at A2602 of 23S rRNA is key to trigger peptidyl-tRNA hydrolysis during translation termination'; Nucl. Acids Res. 2007, 35, 5130-5140.
- R. Rieder, K. Lang, D. Graber, R. Micura*; 'Ligand-Induced Folding of the Adenosine Deaminase A-Riboswitch and Implications on Riboswitch Translational Control'; Chembiochem 2007, 8, 896 - 902.
- R. Micura; 'RNA – Struktur und Funktion'; Trendbericht, Nachrichten aus der Chemie 2007, 55, 279-284.
2006
- R. Micura, C. Höbartner, R. Rieder, C. Kreutz, B. Puffer, K. Lang, H. Moroder; 'Preparation of 2'-Deoxy-2'-Methylseleno-Modified RNA', Curr. Protoc. Nucl. Acid Chem. 2006, Wiley, C1.15.1. - C1.15.34.
- H. Moroder, C. Kreutz, K. Lang, A. Serganov*, and R. Micura*; 'Synthesis, Oxidation Behavior, Crystallization and Structure of 2'-Methylseleno Guanosine Containing RNAs'; J. Am. Chem. Soc. 2006, 128, 9909-9918.
- C. Kreutz, H. Kählig, R. Konrat*, R. Micura*; 'A General Approach for the Identification of Site-Specific RNA Binders by 19F-NMR Spectroscopy - Proof of Concept'; Angew. Chem. 2006, 118, 3528-3532; Angew. Chem. Int. Ed. Engl. 2006, 45, 3450-3453.
- M. D. Erlacher #, K. Lang #, B. Wotzel, R. Rieder, R. Micura*, N. Polacek*; 'Efficient ribosomal peptidyl transfer critically relies on the presence of the ribose 2'-OH at A2451 of 23S rRNA'; J. Am. Chem. Soc. 2006, 128, 4453-4459. # these authors contributed equally
- R. Micura*; 'Programmable ligand-controlled riboregulators'; Angew. Chem. 2006, 118, 32-34; Angew. Chem. Int. Ed. Engl. 2006, 45, 30-31.
2005
- C. Kreutz, H. Kählig, R. Konrat*, R. Micura*, 'Ribose 2'-F labeling: A simple tool for the characterization of RNA secondary structure equilibria by 19F-NMR spectroscopy', J. Am. Chem. Soc. 2005, 127, 11558-11559.
- C. Höbartner, R. Rieder, C. Kreutz, B. Puffer, K. Lang, A. Polonskaia, A. Serganov, R. Micura*, 'Syntheses of RNAs with up to 100 nucleotides containing site-specific 2'-Se-methyl labels for use in X-ray crystallography', J. Am. Chem. Soc. 2005, 127, 12035-12045.
- M. D. Erlacher, K. Lang, N. Shankaran, B. Wotzel, A. Hüttenhofer, R. Micura, A. S. Mankin, N. Polacek*; 'Chemical engineering of the peptidyl transferase center reveals an important role of the 2'-O-hydroxyl group of A2451'; Nucl. Acids Res. 2005, 33, 1618-1627.
- A. Serganov, S. Keiper, L. Malinina, V. Tereshko, E. Skripkin, C. Hobartner, A. Polonskaia, A. T. Phan, R. Wombacher, R. Micura, Z. Dauter, A. Jaschke*, D. J. Patel*; 'Structural basis for Diels-Alder ribozyme-catalyzed carbon-carbon bond formation'; Nat. Struct. Mol. Biol. 2005, 12, 218 - 224.
2004
- A. Serganov, Y. R. Yuan, O. Pikovskaya, A. Polonskaia, L. Malinina, A.T. Phan, C. Höbartner, R. Micura, R.R. Breaker, D.J. Patel*; 'Structural basis for discriminative regulation of gene expression by adenine- and guanine-sensing mRNAs', Chem. Biol. 2004, 11, 1729-1741.
- R. Micura*; 'Genetic control by a natural metabolite-responsive ribozyme'; Angew. Chem. 2004, 116, 4797-4799; Angew. Chem. Int. Ed. Engl. 2004, 43, 4692-4694.
- C. Hoebartner, H. Mittendorfer, K. Breuker, R. Micura*; 'Triggering of RNA secondary structures by a functionalized nucleobase'; Angew. Chem. 2004, 116, 4012-4015; Angew. Chem. Int. Ed. Engl. 2004, 43, 3922-3925.
- C. Hoebartner, R. Micura*; 'The chemical synthesis of selenium-modified oligoribonucleotides and their enzymatic ligation leading to an U6 snRNA stem-loop segment'; J. Am. Chem. Soc. 2004, 126, 1141-1149.
- R. Micura*, C. Hoebartner; 'Equilibria of RNA secondary structures', in Bioorganic Chemistry Highlights II: From Chemistry to Biology, Ed. C. Schmuck, H. Wennemers, WILEY-VCH, Weinheim, 2004, 3-16.
2003
- S. Pitsch, S. Wendeborn, R. Krishnamurthy*, A. Holzner, M. Minton, M. Bolli, C. Miculka, N. Windhab, R. Micura, M. Stanek, B. Jaun, A. Eschenmoser*; 'The ß-D-Ribopyranosyl-(4'-2')-Oligonucleotide System ('Pyranosyl-RNA'): Synthesis and Resume of Base-Pairing Properties'; Helv. Chim. Acta 2003, 12, 4270-4363.
- R. Micura*, and C. Hoebartner; 'On RNA secondary structure rearrangements and equilibria of small RNAs'; ChemBioChem 2003, 4, 984-990.
- C. Hoebartner, C. Kreutz, E. Flecker, E. Ottenschlaeger, W. Pils, K. Grubmayr, R. Micura*; 'The synthesis of 2'-O-[(triisopropylsilyl)oxy]methyl (TOM) phosphoramidites of methylated ribonucleosides (m1G, m2G, m22G, m1I, m3U, m4C, m6A, m62A) for use in automated RNA solid-phase synthesis'; Monatshefte für Chemie - Chemical Monthly, 2003, 134, 851-873.
- C. Hoebartner, R. Micura*; 'Bistable secondary structures of small RNAs and their structural probing by comparative imino proton NMR spectroscopy'; J. Mol. Biol. 2003, 325, 421-431.
2002
- R. Micura*; 'Small interfering RNAs and their chemical synthesis'; Angew. Chem. 2002, 114 (13), 2369-2373; Angew.Chem. Int. Ed. Engl. 2002, 41 (13), 2265-2269.
- C. Hoebartner, M.-O. Ebert, B. Jaun, R. Micura*; 'RNA two-state conformation equilibria and the effect of nucleobase methylation'; Angew. Chem. 2002, 114 (4), 619-623; Angew. Chem. Int. Ed. Engl. 2002, 41 (4), 605-609.
2001
- R. Micura*, W. Pils, C. Hoebartner, K. Grubmayr, M.-O. Ebert, B. Jaun; 'Methylation of nucleobases in RNA oligonucleotides mediates hairpin-duplex conversion'; Nucleic Acids Res. 2001, 29, 3997-4005.
- R. Micura*, W. Pils, K. Grubmayr, M.-O. Ebert, B. Jaun; 'Bridged Cyclic Oligoribonucleotides - towards Models for Codon-Anticodon Pairing'; Nucleosides, Nucleotides, Nucleic Acids 2001, 20, 1287-1289.
2000
- R. Micura*, W. Pils, and K. Grubmayr; 'Bridged Cyclic Oligoribonucleotides as Model Compounds for Codon-Anticodon Pairing'; Angew. Chem. 2000, 112, 956-958; Angew. Chem. Int. Ed. Engl. 2000, 39, 922-925.
- W. Pils and R. Micura*, 'Flexible non-nucleotide linkers as loop replacements in short duplex RNAs'; Nucleic Acids Res. 2000, 28, 1859-1863.
- M.- O. Ebert, Bernhard Jaun* and W. Pils, R. Micura; 'On RNA triplet interactions: NMR study of the short intramolecular duplex formed by (D)-r[GCAm1G-p-O(CH2CH2O)6-p-UGCC]'; Helv. Chem. Acta 2000, 83, 2338-2343.
1999
- R. Micura*; 'Cyclic Oligoribonucleotides (RNA) by Solid Phase Synthesis'; Chemistry Eur. J. 1999, 5, 2077-2082.
- R. Micura, R. Kudick and A. Eschenmoser*; 'Opposite Orientation of Backbone Inclination in pyranosyl-RNA and homo-DNA Correlates with Opposite Directionality of Duplex Properties'; Angew. Chem. 1999, 111, 715-718; Angew. Chem. Int. Ed. Engl. 1999, 38, 680-683
1997
- M. Bolli, R. Micura, S. Pitsch and A. Eschenmoser*; 'Pyranosyl-RNA: Further Observations on Replication'; Helv. Chem. Acta 1997, 80, 1901-1951.
- M. Bolli, R. Micura, and A. Eschenmoser*; 'Pyranosyl-RNA: Chiroselective self-assembly of base sequences by ligative oligomerization of tetranucleotide-2',3'-cyclophosphates (with a commentary concerning the origin of biomolecular homochirality)'; Chem.&Biol. 1997, 4, 309-320.
- R. Micura, M. Bolli, N. Windhab and A. Eschenmoser*; 'Pyranosyl-RNA Also Forms Hairpin Structures', Angew. Chem. 1997, 109, 899-902; Angew. Chem. Int. Ed. Engl. 1997, 36, 870-873.
1996
- R. Micura* and K. Grubmayr; 'The Enantiomers of Phycocyanobilin Dimethyl Ester: A Facile Approach via Semipreparative Enantioselective Chromatography of their Ring-D Methyl Imino Esters'; Enantiomer 1996, 1, 109-113.
- H. Falk*, R. Micura, M. Stanek, and R. Wutka; 'Structural Aspects of Native and Acid or Enzyme Degraded Amylopectins - a 13C NMR Study', Starch/Stärke 1996; 48, 344-346.
- H. Falk*, Q.-Q. Chen, and R. Micura; 'On the Chemistry of Pyrrole Pigments, XCVII: Synthesis, Stereochemistry, and Solvatochromic Effects of a 1-(Dipyrrinon-9-yl)-3-(dipyrrinon-9-ylidene)-1-propene'; Monatsh. 1996, 127, 77-83.
1995
- R. Micura and K. Grubmayr*; 'A Phycocyanobilin Seryliminoester as a New Model for the Chromophore-Protein Interaction in Phytochrome'; Angew. Chem. 1995, 107, 1896-1898; Angew. Chem. Int. Ed. Engl. 1995, 34, 1733-1735.
- Q.-Q. Chen, H. Falk*, and R. Micura; 'On the Chemistry of Pyrrole Pigments, XCII: Syntheses of 1,2-Bis-pyrrolylethanes'; Monatsh. 1995, 126, 473-479.
1994
- R. Micura and K. Grubmayr*; 'Long-wavelength absorbing derivatives of phycocyanobilin: new structural aspects on phytochrome'; Bioorg. Med. Chem. Lett. 1994, 4, 2517-2522.