Introduction Infrared differential thermal analysis (IDTA) and differential imaging chlorophyll fluorescence (DIF) are employed simultaneously to localize ice nucleation and to study the two dimensional pattern of ice propagation in leaves and mesophyll freeze dehydration as detected by a significant increase of basic chlorophyll fluorescence (F0).
Research
Questions
Visualization of ice propagation in plant tissues
Localization of ice nucleation
Rates of ice propagation
Determination of protective ice barriers
Freeze dehydration of mesophyll cells
Publications NEUNER G., PRAMSOHLER M.: Seasonal change of freezing and heat tolerance of PS2 compared to ice nucleation, frost and heat damage in evergreen subalpine plants. Physiologia Plantarum, 126, 196-204 (2006). HACKER, J., NEUNER, G.: Ice propagation in plants visualized at the tissue level by IDTA (infrared differential thermal analysis). Tree Physiology, 27, 1661-1670 (2007). HACKER, J., NEUNER, G.: Ice propagation in dehardened alpine plant species studied by infrared differential thermal analysis (IDTA). Arctic, Antarctic and Alpine Research, 40 (4), 660-670 (2008). HACKER, J., SPINDELBÖCK, J., NEUNER, G.: Mesophyll freezing and effects of freeze dehydration visualized by simultaneous measurement of IDTA and differential imaging chlorophyll fluorescence. Plant, Cell & Environment, 31, 1725-1733 (2008).
