Eduard Stefan, Ph.D.

Eduard Stefan, Ph.D.

Privatdozent Dr.rer.nat.


Institute of Biochemistry
University of Innsbruck
Room no. L.03.042

Center for Chemistry and Biomedicine (CCB) Innrain 80-82, 6020 Innsbruck / Austria
Phone: +43 512 507 - 57531 | Fax: +43 512 507 - 57599



Enzler F, Tschaikner P, Schneider R, Stefan E*. KinCon: Cell-based recording of full-length kinase conformations. IUBMB Life, in press (2020).


Tschaikner P, Enzler F, Torres-Quesada O, Aanstad P, Stefan E*. Hedgehog and Gpr161: Regulating cAMP Signaling in the Primary Cilium. Cells, 9, 118 (2020).


Ortner NJ, Pinggera A, Hofer NT, Siller A, Brandt N, Raffeiner A, Vilusic K, Lang I, Blum K, Obermair GJ, Stefan E, Engel J and Striessnig J. RBP2 stabilizes slow Cav1.3 Ca(2+) channel inactivation properties of cochlear inner hair cells. Pflugers Arch. 472(1):3-25 (2020).


Röck R, Mayrhofer JE, Torres-Quesada O, Enzler F, Raffeiner A, Raffeiner P, Feichtner A, Huber RG, Koide S, Taylor SS, Troppmair J, Stefan E*. BRAF inhibitors promote intermediate BRAF(V600E) conformations and binary interactions with activated RAS. Science Advances, in press (2019).


Rinaldi L, Donne R, Catalanotti B, Torres-Quesada O, Moraca F, Nisticò R, Chiuso F, Piccinin S, Enzler F, Bachmann V, Garbi C, Scorziello A, Russo N, Synofzik M, Stelzl U, Annunziato L, Stefan E, Feliciello A. Feedback inhibition of cAMP effector signaling by a chaperone-assisted ubiquitin system. Nat. Commun., 10(1):2572. (2019)


Porpora M, Sauchella S, Rinaldi L, Delle Donne R, Sepe M, Torres-Quesada O, Intartaglia D, Garbi C, Insabato L, Santoriello M, Bachmann V, Synofzik M, Lindner H, Ivan C, Stefan E, Feliciello A. Counterregulation of cAMP-directed kinase activities controls ciliogenesis. Nat. Commun. 9, e1224 (2018).


Stefan E*, Troppmair J, Bister K. Targeting the architecture of deregulated protein complexes in cancer. Adv. Protein Chem. Struct. Biol., 111:101-132 (2018).


Haushalter K, Casteel DE, Raffeiner A, Stefan E, Patel HH, Taylor SS. Phosphorylation of Protein Kinase A (PKA) regulatory subunit RIa by Protein Kinase G (PKG) primes PKA for catalytic activity in cells. J Biol Chem. 293, 4411-4421 (2018).


Torres-Quesada O, Mayrhofer JE, Stefan E*. The many faces of compartmentalized PKA signalosomes. Cell. Signal. 37, 1-11 (2017).


Stefan E, Bister K. MYC and RAF: Key effectors in cellular signaling and major drivers in human cancer. Curr. Top. Microbiol. Immunol. 407, 117-151 (2017).


Raffeiner P, Schraffl A, Schwarz T, Röck R, Ledolter K, Hartl M, Konrat R, Stefan E*, Bister K*. Calcium-dependent binding of Myc to calmodulin. Oncotarget 8, 3327-3343 (2017).


Torres-Quesada O, Röck R, Stefan E*. Systematic quantification of cAMP-controlled PKA interactions. Hormone and Metabolic Research 49, 240-249 (2017).


Bruystens JG, Wu J, Fortezzo A, Del Rio J, Nielsen C, Blumenthal DK, Rock R, Stefan E, Taylor SS. Structure of a PKA RIα Recurrent Acrodysostosis Mutant Explains Defective cAMP-Dependent Activation. J. Mol. Biol. 428, 4890-4904 (2016).


Bachmann VA, Mayrhofer JE, Ilouz R, Tschaikner PM, Raffeiner P, Röck R, Courcelles M, Apelt F, Lu T, Baillie GS, Thibault P, Aanstad P, Stelzl U, Taylor SS, Stefan E*. Gpr161 anchoring of PKA consolidates GPCR and cAMP signaling. Proc. Natl. Acad. Sci. USA 113, 7786-7791 (2016).


Rinaldi L, Delle Donne R, Sepe M, Porpora M, Garbi C, Chiuso F, Gallo A, Parisi S, Russo L, Bachmann V, Huber RG, Stefan E, Russo T, Feliciello A. praja2 regulates KSR1 stability and mitogenic signaling. Cell Death Dis. 7:e2230 (2016).


Röck R, Mayrhofer JE, Bachmann V, Stefan E*. Impact of kinase activating and inactivating patient mutations on binary PKA interactions. Front. Pharmacol. 6:170 (2015).


Stefan E, Hart JR, Bister K. Stopping MYC in its tracks. Aging 7, 463-464 (2015).


Röck R, Bachmann V, Bhang HC, Malleshaiah M, Raffeiner P, Mayrhofer JE, Tschaikner PM, Bister K, Aanstad P, Pomper MG, Michnick SW, Stefan E*. In-vivo detection of binary PKA network interactions upon activation of endogenous GPCRs. Sci. Rep. 5, 5:11133 (2015).


Filteau M, Diss G, Torres-Quiroz F, Dubé AK, Schraffl A, Bachmann VA, Gagnon-Arsenault I, Chrétien AÈ, Steunou AL, Dionne U, Côté J, Bisson N, Stefan E, Landry CR. Systematic identification of signal integration by protein kinase A. Proc. Natl. Acad. Sci. USA. 112, 4501-4506 (2015).


Raffeiner P, Röck R, Schraffl A, Hartl M, Hart JR, Janda KD, Vogt PK, Stefan E*, Bister K*. In vivo quantification and perturbation of Myc-Max interactions and the impact on oncogenic potential. Oncotarget 5, 8869-8878 (2014).


Hart JR, Garner AL, Yu J, Ito Y, Sun M, Ueno L, Rhee J-K, Baksh MM, Stefan E, Hartl M, Bister K, Vogt PK, Janda KD. Inhibitor of MYC identified in a Kröhnke pyridine library. Proc. Natl. Acad. Sci. USA 111, 12556-12561 (2014).


Bachmann VA, Bister K, Stefan E*. Interplay of PKA and Rac: Fine-tuning of Rac localization and signaling. Small GTPases 4, 247-251 (2013).


Lignitto L, Arcella A, Sepe M, Rinaldi L, Delle Donne R, Gallo A, Stefan E, Bachmann VA, Oliva MA, Tiziana Storlazzi C, L'abbate A, Brunetti A, Gargiulo S, Gramanzini M, Insabato L, Garbi C, Gottesman ME, Feliciello A. Proteolysis of MOB1 by the ubiquitin ligase praja2 attenuates Hippo signalling and supports glioblastoma growth. Nat. Commun. 4, 1822 (2013).


Bachmann VA, Riml A, Huber RG, Baillie GS, Liedl KR, Valovka T, Stefan E*. Reciprocal regulation of PKA and Rac signaling. Proc. Natl. Acad. Sci. USA 110, 8531-8536 (2013).


Stückemann T, Wegleiter T, Stefan E, Nägele O, Tarbashevich K, Böck G, Raz E, Aanstad P. Zebrafish Cxcr4a determines the proliferative response to Hedgehog signalling. Development 139, 2711-2720 (2012).


Stefan E*, Malleshaiah MK, Breton B, Ear PH, Bachmann V, Beyermann M, Bouvier M, Michnick SW. PKA regulatory subunits mediate synergy among conserved GPCR cascades. Nat. Commun. 2, 598 (2011).


Lignitto L, Carlucci A, Sepe M, Stefan E, Cuomo O, Nistico R, Scorziello A, Savoia C, Garbi C, Annunziato L, Feliciello A. Control of PKA stability and signalling by RING ligase praja2. Nat. Cell Biol. 13, 412-422 (2011).


Skoumbourdis AP, Leclair CA, Stefan E, Turjanski AG, Maguire W, Titus SA, Huang R, Auld DS, Inglese J, Austin CP, Michnick SW, Xia M, Thomas CJ. Exploration and optimization of substituted triazolothiadiazines and triazolopyridazines as PDE4 inhibitors. Bioorg. Med. Chem. Lett. 19, 3686-3692 (2009).


Nyfeler B, Reiterer V, Wendeler MW, Stefan E, Zhang B, Michnick SW, Hauri HP. Identification of ERGIC-53 as an intracellular transport receptor of alpha1-antitrypsin. J. Cell Biol. 180, 705-712 (2008).


Stefan E, Aquin S, Berger N, Landry CR, Nyfeler B, Bouvier M, Michnick SW. Quantification of dynamic protein complexes using Renilla luciferase fragment complementation applied to protein kinase A activities in vivo. Proc. Natl. Acad. Sci. USA 104, 16916-16921 (2007).


Michnick SW, Ear PH, Manderson EN, Remy I, Stefan E. Universal strategies in research and drug discovery based on protein-fragment complementation assays. Nat. Rev. Drug Discov. 6, 569-582 (2007).


Nedvetsky PI, Stefan E, Frische S, Santamaria K, Wiesner B, Valenti G, Hammer JA 3rd, Nielsen S, Goldenring JR, Rosenthal W, Klussmann E. A role of myosin Vb and Rab11-FIP2 in the aquaporin-2 shuttle. Traffic 8, 110-123 (2007).


Stefan E, Wiesner B, Baillie GS, Mollajew R, Henn V, Lorenz D, Furkert J, Santamaria K, Nedvetsky P, Hundsrucker C, Beyermann M, Krause E, Pohl P, Gall I, MacIntyre AN, Bachmann S, Houslay MD, Rosenthal W, Klussmann E. Compartmentalization of cAMP-dependent signaling by phosphodiesterase-4D is involved in the regulation of vasopressin-mediated water reabsorption in renal principal cells. J. Am. Soc. Nephrol. 18, 199-212 (2007).


McSorley T, Stefan E, Henn V, Wiesner B, Baillie GS, Houslay MD, Rosenthal W, Klussmann E. Spatial organisation of AKAP18 and PDE4 isoforms in renal collecting duct principal cells. Eur. J. Cell Biol. 85, 673-678 (2006).


Hundsrucker C, Krause G, Beyermann M, Prinz A, Zimmermann B, Diekmann O, Lorenz D, Stefan E, Nedvetsky P, Dathe M, Christian F, McSorley T, Krause E, McConnachie G, Herberg FW, Scott JD, Rosenthal W, Klussmann E. High-affinity AKAP7 delta-protein kinase A interaction yields novel protein kinase A-anchoring disruptor peptides. Biochem. J. 396, 297-306 (2006).


Milojevic T, Reiterer V, Stefan E, Korkhov VM, Dorostkar MM, Ducza E, Ogris E, Boehm S, Freissmuth M, Nanoff C. The ubiquitin-specific protease Usp4 regulates the cell surface level of the A2A receptor. Mol. Pharmacol. 69, 1083-1094 (2006).


Henn V, Stefan E, Baillie GS, Houslay MD, Rosenthal W, Klussmann E. Compartmentalized cAMP signalling regulates vasopressin-mediated water reabsorption by controlling aquaporin-2. Biochem. Soc. Trans. 33, 1316-1318 (2005).


Gsandtner I, Charalambous C, Stefan E, Ogris E, Freissmuth M, Zezula J. Heterotrimeric G protein-independent signaling of a G protein-coupled receptor. Direct binding of ARNO/cytohesin-2 to the carboxyl terminus of the A2A adenosine receptor is necessary for sustained activation of the ERK/MAP kinase pathway. J. Biol. Chem. 280, 31898-31905 (2005).


Henn V, Edemir B, Stefan E, Wiesner B, Lorenz D, Theilig F, Schmitt R, Vossebein L, Tamma G, Beyermann M, Krause E, Herberg FW, Valenti G, Bachmann S, Rosenthal W, Klussmann E. Identification of a novel A-kinase anchoring protein 18 isoform and evidence for its role in the vasopressin-induced aquaporin-2 shuttle in renal principal cells. J. Biol. Chem. 279, 26654-26665 (2004).


Klinger M, Kuhn M, Just H, Stefan E, Palmer T, Freissmuth M, Nanoff C. Removal of the carboxy terminus of the A2A-adenosine receptor blunts constitutive activity: Differential effect on cAMP accumulation and MAP kinase stimulation. Naunyn-Schmiedebergs Arch. Pharmacol. 366, 287-298 (2002).


Just H, Stefan E, Czupalla C, Nurnberg B, Nanoff C, Freissmuth M. Beyond G proteins: The role of accessory proteins in G protein-coupled receptor signalling. In Trends in Drug Research III, Pharmacochemistry Library Vol. 32 (van der Goot H, ed), pp. 161-174. Elsevier, Amsterdam (2002).

Patent Applications

  1. Stefan E*, Bachmann V, Huber R. Peptidic inhibitor of signal transmission from Gαs to Gαi-coupled receptor cascades. European Patent Office, PCT/EP2012/074489 (2013); United States Patent Application No.: 14/366874 (2015) 

  2. Stefan E*, Huber R, Bachmann V, Liedl KR.  Bioactive small molecules modulate GPCR cross talk  (07/2012) Submission number K120026 (AWS reference: P1204009); Cast Tirol, University of Innsbruck, Innsbruck, Austria 

  3. Stefan E*, Röck R, Raffeiner P, Bachmann V. Intermolecular kinase reporter (submission 05/2016), EU patent application

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